Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems

Patent metadata

Field	Value
Publication number	US-9340416-B2
Application number	US-54005209-A
Country	US
Kind code	B2
Filing date	Aug 12, 2009
Priority date	Aug 13, 2008
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

A linker polynucleotide for attaching a nanomaterial to a polynucleotidic platform and related nanoassemblies, arrangements, structures, methods and systems.

First claim

What is claimed is:

1. A structure comprising a linker polynucleotide attached both to a nonomaterial and to a polynucleotidic platform, the linker polynucleotide comprising:
a toehold moiety specifically attached to a corresponding moiety presented on the polynucleotidic platform;
a duplex protected labeling polynucleotide domain; and
a nanomaterial attaching moiety comprising a poly T dispersal domain directly physisorbed on the nanomaterial,
wherein the nanomaterial attaching moiety and the toehold moiety are on a same strand of the linker polynucleotide, and
wherein the duplex protected labeling polynucleotide domain is configured to allow deprotection and subsequent binding upon binding of the toehold moiety to the platform.

2. The structure of claim 1 , wherein the linker polynucleotide is made of DNA, nucleic acid analogues, or a combination of DNA and nucleic acid analogues.

3. The structure of claim 2 , wherein the nucleic acid analogues comprise one or more of LNA and PNA.

4. The structure of claim 1 , wherein the duplex protected labeling polynucleotide domain has a length of at least ten base pairs.

5. The structure of claim 1 , wherein the toehold moiety is a single-stranded polynucleotide region and the corresponding moiety presented on the polynucleotidic platform is a complementary single-stranded polynucleotide region.

6. The structure of claim 5 , wherein the single-stranded polynucleotide region of the toehold moiety is a hairpin loop.

7. The structure of claim 1 , wherein the toehold moiety comprises a chemical linker moiety configured to specifically attach a corresponding moiety presented on the polynucleotidic platform.

8. The structure of claim 1 , wherein the polynucleotidic platform comprises,
a polynucleotide template extending along a template plane; and
a hook polynucleotide arranged on the polynucleotidic template and comprising a linker polynucleotide attaching moiety presented on the polynucleotidic platform,
wherein the linker polynucleotide attaching moiety of the hook polynucleotide is the corresponding moiety attached to the toehold moiety of the linker polynucleotide.

9. The structure of claim 8 , wherein the hook polynucleotide is projecting out of the template plane.

10. The structure of claim 8 , wherein the linker polynucleotide attaching moiety of the hook polynucleotide is spaced from a polynucleotidic template binding moiety of the hook polynucleotide.

11. The structure of claim 1 , wherein the polynucleotidic platform comprises
a polynucleotide template extending along a template plane, and
one or more hook polynucleotides arranged on the template plane to present a linker polynucleotide attaching moiety specifically attached to a corresponding toehold moiety of the linker polynucleotide, and

wherein the polynucleotide template is a DNA origami, a DNA ribbon or a nanoscale polyhedral.

12. The structure of claim 11 , wherein the structure is a three dimensional assembled structure.

13. The structure of claim 11 , wherein the structure is a cross junction, a field effect transistors or a sandwiched 3D device stacks with layers of origami stacked together on top of each other.

14. The structure of claim 11 , wherein the nanomaterial comprises single wall carbon nanotubes.

15. The structure of claim 1 , wherein the nanomaterial comprises single wall carbon nanotubes.

16. A nanoassembly comprising:
structure of claim 1 ,

wherein the nanomaterial is selected from carbon spheres, carbon nanotubes and carbon nanowires.

17. A method of organizing a nanomaterial, the method comprising;
contacting a polynucleotidic platform with a nanoassembly to form a structure comprising the nanoassembly attached to the polynucleotidic platform;
wherein
the polynucleotidic platform comprises a polynucleotide template extending along a template plane; and one or more hook polynucleotides arranged on the template plane;

wherein
the nanoassembly comprises a nanomaterial and a linker polynucleotide, the linker polynucleotide comprising:
a toehold moiety configured to specifically attach one or more corresponding linker polynucleotide attaching moiety presented on the one or more hook polynucleotides;
a duplex protected labeling polynucleotide domain configured to allow deprotection and subsequent binding upon binding of the toehold moiety to the platform; and
a nanomaterial attaching moiety comprising a poly T dispersal domain directly physisorbed on the nanomaterial;

with the nanonmaterial attaching moiety and the toehold moiety on a same strand of the linker polynucleotide,
and wherein
contacting the polynucleotide platform with the nanoassembly is performed for a time and under condition to form the structure of claim 1 by specific binding of the toehold moiety of the linker polynucleotide of the nanoassembly with the linker polynucleotide attaching moiety of the one or more hook polynucleotides.

18. A structure comprising a linker polynucleotide attached both to a nanomaterial and to a polynucleotidic platform, the linker polynucleotide comprising
a toehold moiety formed by a polynucleotide hairpin loop specifically attached to a corresponding single-stranded polynucleotide moiety presented on the polynucleotidic platform;
a duplex protected labeling polynucleotide domain; and
a nanomaterial attaching moiety directly adsorbed on the nanomaterial via physisorption.

19. A structure comprising a linker polynucleotide attached both to a nanomaterial and to a polynucleotidic platform, the linker polynucleotide comprising
a toehold moiety specifically attached to a corresponding moiety presented on the polynucleotidic platform;
a duplex protected labeling polynucleotide domain; and
a nanomaterial attaching moiety comprising a poly T dispersal domain directly physisorbed on the nanomaterial,
wherein the nanomaterial attaching moiety and the toehold moiety are on a same strand of the linker polynucleotide
wherein the toehold moiety is a single-stranded polynucleotide region and the corresponding moiety presented on the polynucleotidic platform is a complementary single-stranded polynucleotide region and
wherein the single-stranded polynucleotide region of the toehold moiety is a hairpin loop.

20. The structure of claim 19 , wherein the linker polynucleotide is made of DNA, nucleic acid analogues, or a combination of DNA and nucleic acid analogues.

21. The structure of claim 20 , wherein the nucleic acid analogues comprise one or more of LNA and PNA.

22. The structure of claim 19 , wherein the duplex protected labeling polynucleotide domain has a length of at least ten base pairs.

23. The structure of claim 19 , wherein the nanomaterial is carbon spheres, carbon nanotubes or carbon nanowires.

24. The structure of claim 19 , wherein the nanomaterial comprises single wall carbon nanotubes.

25. The structure of claim 19 , wherein the polynucleotidic platform comprises a polynucleotide template selected from a DNA origami, a DNA ribbon, and a nanoscale polyhedral.

Assignees

• Maune Hareem T
• Han Si-Ping
• Barish Robert D
• Bockrath Marc W
• Goddard Iii William A
• Rothemund Paul W K
• Winfree Erik
• California Inst Of Techn

Inventors

• Maune Hareem T
• Han Si-Ping
• Barish Robert D
• Bockrath Marc W
• Goddard Iii William A
• Rothemund Paul W K
• Winfree Erik

Classifications

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• B82B1/00 Primary

  Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units · CPC title