Chemically Modified Graphene

Patent metadata

Field	Value
Publication number	US-9339790-B2
Application number	US-201214118596-A
Country	US
Kind code	B2
Filing date	May 18, 2012
Priority date	May 19, 2011
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

This disclosure relates to graphene derivatives, as well as related devices including graphene derivatives and methods of using graphene derivatives.

First claim

What is claimed is:

1. A material, comprising:
a graphene derivative comprising a graphene core and a first pendant group, the first pendant group comprising a metal ion and a linking group wherein the metal ion is complexed with the linking group and the linking group is covalenty bonded to the graphene core.

2. The material of claim 1 , wherein the linking group comprises a nitrilotriacetic acid moiety, a tris-nitrilotriacetic acid moiety, or an iminodiacetic acid moiety.

3. The material of claim 1 , wherein the linking group is covalently bonded to the graphene core through an amide group.

4. The material of claim 1 , wherein the first pendant group further comprises a His-tagged protein binding to the metal ion.

5. The material of claim 4 , wherein the His-tagged protein is His-tagged Protein A or His-tagged Protein G.

6. The material of claim 1 , wherein the metal ion is Ni 2+ , Cu 2+ , Zn 2+ , Ag + , Fe 3+ , Ga 3+ , Zr 3+ , Ca 2+ , or Co 2+ .

7. A material, comprising:
a graphene derivative comprising a graphene core and a first pendant group, the first pendant group comprising a SiO 2 nanoparticle covalently bonded to the graphene core through an amide group.

8. The material of claim 7 , wherein the SiO 2 nanoparticle is covalently bonded to the graphene core through a linkage —CO—NH—R—Si(—O—) 3 , in which R is C 1 -C 20 alkylene.

9. The material of claim 7 , wherein the graphene derivative further comprises a second pendant group, the second pendant group comprising metal ion or a TiO 2 nanoparticle.

10. A chromatography column, comprising a column made from metal, glass, or plastic packed with a material comprising a graphene derivative comprising a graphene core and a first pendant group, the first pendant group comprising a metal ion, a nanoparticle, a sulfonate group, an amine group, a quaternary ammonium group, or a chelating group wherein the first pendant group is attached to the graphene core covalenty, or complexed ionically or coordinatively to one or more linking group covalently bonded to the graphene core.

11. The column of claim 10 , wherein the column further comprises a spacer mixed with the material.

12. The column of claim 11 , wherein the spacer is an oxide or a polymer.

13. A chromatographic system, comprising the chromatography column of claim 10 .

14. A method of isolating a protein from a sample, comprising:
disposing the sample in a dispersion comprising a material comprising a graphene derivative comprising a graphene core and a first pendant group, the first pendant group comprising a metal ion, a nanoparticle, a sulfonate group, an amine group, a quaternary ammonium group, a chelating group, or a protein wherein the first pendant group is attached to the graphene core covalenty, or complexed ionically or coordinatively to one or more linking group covalently bonded to the graphene core,
thereby binding the protein to be isolated to the material to form a protein-bound material;
isolating the protein-bound material; and
recovering the protein from the protein-bound material.

15. The method of claim 14 , wherein isolating the protein-bound material is carried out by centrifugation, filtration, or decantation.

16. The method of claim 14 , wherein the first pendant group comprises a metal ion.

17. The method of claim 16 , wherein the protein is a His-tagged protein, a FLAG-tagged protein, a HA-tagged protein, a myc-tagged protein, a GST-tagged protein, a MBP-tagged protein, or a lectin.

18. The method of claim 17 , wherein the His-tagged protein is a His-tagged photosystem I core complex, a His-tagged photosystem II core complex, or a His-tagged bacterial reaction center.

19. The method of claim 14 , wherein the first pendant group comprises a TiO 2 nanoparticle.

20. The method of claim 19 , wherein the protein is a phosphoprotein.

21. The method of claim 20 , wherein the phosphoprotein is an Fe receptor, a Ulk antibody, a calcineurin, a K chromatin immunoprecipitate, or a urocotin.

22. The method of claim 14 , wherein the first pendant group comprises a protein.

23. The method of claim 22 , wherein the protein in the first pendant group comprises Protein A, Protein G, or an E72G3 protein.

24. The method of claim 23 , wherein the protein in the sample is an antibody having an Fe region.

Assignees

• Vittadello Michele
• Woronowicz Kamil
• Chhowalla Manish
• Falkowski Paul G
• Harrold Jr John W
• Univ City New York Res Found
• Univ Rutgers

Inventors

• Vittadello Michele
• Woronowicz Kamil
• Chhowalla Manish
• Falkowski Paul G
• Harrold Jr John W

Classifications

• G01N33/551 Primary

  the carrier being inorganic · CPC title

• C07K14/315

  from Streptococcus (G), e.g. Enterococci · CPC title

• B01J20/3206

  Organic carriers, supports or substrates · CPC title

• Y02E50/17

  Cross-Sectional Technologies · mapped topic

• B01J20/3265

  with an organic functional group containing a metal, e.g. a metal affinity ligand · CPC title