Photosensitizer-metal nanoparticle complex and composition containing the complex for photodynamic therapy or diagnosis

Patent metadata

Field	Value
Publication number	US-9339557-B2
Application number	US-66501309-A
Country	US
Kind code	B2
Filing date	Oct 8, 2009
Priority date	Oct 8, 2009
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

Provided are a photosensitizer-metal nanoparticle complex and a composition for photodynamic therapy or diagnosis having the same. The complex includes a photosensitizer, a metal nanoparticle, and a backbone linking the photosensitizer with the metal nanoparticle. The backbone has a polypeptide substrate capable of being specifically degraded by a protease. When the complex is administered to a patient, fluorescence and production of reactive oxygen species from the conjugated photosensitizers are inhibited in normal tissues due to the resonance energy transfer between the photosensitizer and metal nanoparticles, but in tumor tissues, fluorescence and production of reactive oxygen species from the released photosensitizers are activated, thereby effectively destroying the tumor tissues. In addition, the selective fluorescence in the tumor tissues can further improve accuracy of tumor diagnosis using the protease-activatable photosensitizer-metal nanoparticle complex.

First claim

The invention claimed is:

1. A photosensitizer-metal nanoparticle complex, which is represented by the following Formula (“Pro-Leu-Gly-Val-Arg-Gly-Lys” disclosed as SEQ ID NO: 17):

wherein M is a metal nanoparticle, PS is a photosensitizer having a carboxyl group, and
the lysine (Lys) is linked with the photosensitizer by an amide bond.

2. The complex according to claim 1 , wherein the photosensitizer is a porphyrin-based compound or a non-porphyrin compound in the form of a free base or metal complex.

3. The complex according to claim 2 , wherein the photosensitizer is pyropheophorbide a.

4. The complex according to claim 1 , wherein the metal nanoparticle has gold, silver, copper, platinum, palladium, nickel, iron, or a combination of at least two thereof.

5. The complex according to claim 1 , wherein the metal nanoparticle is a gold nanoparticle.

6. The complex according to claim 1 , wherein the metal nanoparticle has the form of a sphere, rod, pyramid, star, or core-shell.

7. A composition for photodynamic therapy or diagnosis, comprising a photosensitizer-metal nanoparticle complex according to claim 1 and a pharmaceutically available carrier.

8. A photosensitizer-metal nanoparticle complex, which is represented by Formula 1 (“Pro-Leu-Gly-Val-Arg-Gly” disclosed as SEQ ID NO: 15):

Assignees

• Choi Yong-Doo
• Jang Bo-Seung
• Kim In-Hoo
• Park Jin-Young
• Nat Cancer Ct

Inventors

• Choi Yong-Doo
• Jang Bo-Seung
• Kim In-Hoo
• Park Jin-Young

Classifications

• A61K49/0017

  Fluorescence in vivo · CPC title

• A61K49/0065

  the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle · CPC title

• A61K41/0071

  PDT with porphyrins having exactly 20 ring atoms, i.e. based on the non-expanded tetrapyrrolic ring system, e.g. bacteriochlorin, chlorin-e6, or phthalocyanines · CPC title

• A61K47/6923

  the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb · CPC title

• A61P35/00

  Antineoplastic agents · CPC title