Hydrolase protein
US-9029107-B2 · May 12, 2015 · US
Hydrolase protein
US9334509B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9334509-B2 |
| Application number | US-201514637429-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 4, 2015 |
| Priority date | Aug 31, 2010 |
| Publication date | May 10, 2016 |
| Grant date | May 10, 2016 |
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- Title
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Abstract
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It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1 -dicarboxylate.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for producing (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid, which comprises contacting dialkyl 2-vinylcyclopropane-1,1-dicarboxylate with a hydrolase protein to produce (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid, wherein the hydrolase protein is capable of catalyzing the following reaction at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO: 1: comprises an amino acid sequence shown in any one of SEQ ID NOs: 2 to 5. 2. A method for producing (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid, which comprises contacting dialkyl 2-vinylcyclopropane-1,1-dicarboxylate with a hydrolase protein to produce (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid, wherein the hydrolase protein consists of an amino acid sequence shown in any one of SEQ ID NOs: 2 to 5.
Assignees
Inventors
Classifications
- C12P7/62Primary
Carboxylic acid esters · CPC title
Carboxylic ester hydrolases {(3.1.1)} · CPC title
by esterification of carboxylic acid groups in the enantiomers or the inverse reaction · CPC title
using catalysts, e.g. selective catalysts · CPC title
Hydrolases (3) · CPC title
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- What does patent US9334509B2 cover?
- It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a …
- Who is the assignee on this patent?
- Api Corp
- What technology area does this patent fall under?
- Primary CPC classification C12P7/62. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 10 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).