Fluorochromes for organelle tracing and multi-color imaging

Patent metadata

Field	Value
Publication number	US-9334281-B2
Application number	US-23198808-A
Country	US
Kind code	B2
Filing date	Sep 8, 2008
Priority date	Sep 8, 2008
Publication date	May 10, 2016
Grant date	May 10, 2016

Abstract

Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest.

First claim

What is claimed is:

1. A compound selected from:

2. The compound having the structure

3. A multimeric compound comprising two or more of the compounds of claim 1 or 2 joined together through a linkage group comprising a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted heteroalkyl group, a substituted or unsubstituted heteroalkenyl group comprising an amide, or one or more substituted or unsubstituted aromatic rings.

4. A compound comprising two organelle-targeting fluorochrome compounds having the structure:

wherein R 1 -R 4 and R 5 -R 8 are independently selected from
H,
OH,
a halide,
a nitro group,
a substituted amine,
a CN group,
a charged group comprising a salt of an organic acid, an onium group or a protonated amine, wherein
said salt of an organic acid comprises a sulfate, a sulfonate, a phosphate, a phosphonate, a carboxylate, a borate, or a combination thereof, and
said onium group comprises a quaternary ammonium, a sulfonium, a phosphonium, or a combination thereof,

a substituted or unsubstituted alkyl or alkenyl group,
a substituted or unsubstituted amine, and
a thiourea group;
wherein only one member of R 1 -R 8 comprises the structure NH-A-R a R b , wherein A is a C 2-8 alkylene group and R a and R b are independently selected from H, a C 1-4 alkyl, a C 2-4 hydroxyalkyl and a C 2-4 aminoalkyl; and
wherein said two or more compounds are joined together through a linkage group comprising a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted heteroalkyl group, a substituted or unsubstituted heteroalkenyl group comprising an amide, or one or more substituted or unsubstituted aromatic rings.

5. The compound of claim 4 selected from

6. A compound selected from the group consisting of

Assignees

• Patton Wayne Forrest
• Pande Praveen
• Xiang Yuejun
• Li Zaiguo
• Donegan James J
• Rabbani Elazar
• Enzo Life Sciences Inc

Inventors

• Patton Wayne Forrest
• Pande Praveen
• Xiang Yuejun
• Li Zaiguo
• Donegan James J
• Rabbani Elazar

Classifications

• C07C335/20

  being further substituted by nitrogen atoms, not being part of nitro or nitroso groups · CPC title

• C07D213/74

  Amino or imino radicals substituted by hydrocarbon or substituted hydrocarbon radicals · CPC title

• C09B1/005

  Di-anthraquinonyl and derivative compounds · CPC title

• C07F9/65038

  condensed with carbocyclic rings or carbocyclic ring systems · CPC title

• C07C229/18

  the nitrogen atom of the amino group being further bound to carbon atoms of six-membered aromatic rings · CPC title