Container storing freeze-dried biological sample

Patent metadata

Field	Value
Publication number	US-9329112-B2
Application number	US-201213982312-A
Country	US
Kind code	B2
Filing date	Jan 25, 2012
Priority date	Jan 28, 2011
Publication date	May 3, 2016
Grant date	May 3, 2016

Abstract

A container holding, and a method of storing, freeze-dried biological samples. The container includes a chamber having an upper portion and a lower portion. The chamber includes a wall, and the lower portion of the chamber is fluidly connected to the upper portion of the chamber such that, when liquid is received at the upper portion, the received liquid can pass to and accumulate in the lower portion. Further, the freeze-dried material is located in the lower portion, and the container includes a physical structure in the form of a stop protruding inwards from the wall, the physical structure being for inhibiting the freeze-dried material from moving from the lower portion of the chamber to the upper portion of the chamber.

First claim

The invention claimed is:

1. A container storing a freeze-dried material comprising a biological sample, the container comprising a chamber having an upper portion and a lower portion, the chamber comprising a wall and the lower portion being fluidly connected to the upper portion such that, when liquid is received at the upper portion, the received liquid can pass to and accumulate in the lower portion,
wherein the freeze-dried material is located in said lower portion, and the container comprises a physical structure protruding inwards from the wall, the physical structure being for inhibiting the freeze-dried material from moving from the lower portion of the chamber to the upper portion of the chamber,
wherein the freeze-dried material defines a first cross-section and the physical structure comprises a stop extending inwards from an internal wall of said chamber, thereby defining a second cross-section, the second cross-section having a dimension smaller than a corresponding dimension of the first cross-section, and
wherein the biological sample is an enzyme configured for nucleic acid amplification.

2. The container of claim 1 , wherein the stop defines a boundary between the upper portion of the chamber and the lower portion of the chamber.

3. The container of claim 2 , wherein the stop comprises a movable insert, the movable insert being movable within said chamber, thereby varying said boundary.

4. The container of claim 1 , wherein the stop comprises a collar integrally formed with said chamber.

5. The container of claim 1 , wherein said physical structure comprises a textured surface.

6. The container of claim 5 , further comprising a plurality of fins extending inwards from the wall of the chamber.

7. The container of claim 1 , wherein the container is of a plastics material.

8. The container of claim 1 , wherein the chamber has a substantially circular cross-section.

9. The container of claim 1 , wherein said upper portion extends from a first end of the container, and the container comprises a seal at the first end.

10. The container of claim 1 , wherein the enzyme is a polymerase.

11. An apparatus storing a freeze dried material comprising a biological sample, the apparatus comprising:
a plurality of containers according to any preceding claim; and
a base plate supporting the plurality of containers.

12. The apparatus of claim 11 , wherein the base plate is of a plastics material.

13. A method of storing a biological sample, comprising:
providing a container, the container comprising a chamber having an upper portion and a lower portion, the chamber having a wall and the lower portion being fluidly connected to the upper portion such that, when liquid is received at the upper portion, the received liquid can pass to and accumulate in the lower portion;
inserting a material containing a biological sample into the chamber and accumulating the material in the lower portion of the chamber; and
performing a freeze-drying process on the material, whereby a freeze-dried material comprising said biological sample is formed in the lower portion,
wherein the container comprises a physical structure protruding inwards from the wall, the physical structure being for inhibiting the freeze-dried material from moving from the lower portion to the upper portion,
wherein the freeze-dried material defines a first cross-section and the physical structure comprises a stop extending inwards from an internal wall of said chamber, thereby defining a second cross-section, the second cross-section having a dimension smaller than a corresponding dimension of the first cross-section, and
wherein the biological sample is an enzyme configured for nucleic acid amplification.

14. The method of claim 13 , wherein the enzyme is a polymerase.

15. The method of claim 13 , wherein the container further comprises a plurality of fins extending inwards from the wall of the chamber, and wherein the plurality of fins and the physical structure comprise a textured surface.

16. A container storing a freeze-dried material comprising a biological sample, the container comprising a chamber having an upper portion and a lower portion, the chamber comprising a wall and the lower portion being fluidly connected to the upper portion such that, when liquid is received at the upper portion, the received liquid can pass to and accumulate in the lower portion,
wherein the freeze-dried material is located in said lower portion, and the wall in said lower portion comprises a rough surface to which the freeze-dried material is bonded, whereby the freeze-dried biological sample is prevented from moving from the lower portion to the upper portion, and
wherein the biological sample is an enzyme configured for nucleic acid amplification.

17. The container of claim 16 , wherein the enzyme is a polymerase.

Assignees

• Smith Michael John
• Tortorella Stevan Paul
• Ge Healthcare Uk Ltd

Inventors

• Smith Michael John
• Tortorella Stevan Paul

Classifications

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• G01N1/42 Primary

  Low-temperature sample treatment, e.g. cryofixation · CPC title

• B01L2300/06

  Auxiliary integrated devices, integrated components · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title