Flow passage device and testing system using the same

Patent metadata

Field	Value
Publication number	US-9328380-B2
Application number	US-201214116088-A
Country	US
Kind code	B2
Filing date	Apr 23, 2012
Priority date	May 10, 2011
Publication date	May 3, 2016
Grant date	May 3, 2016

Abstract

A real time analysis in accordance with temperature change and highly sensitive detection are permitted. A flow passage device has a flow passage ( 1 ) and is able to heat a fluid in the flow passage. A state of the fluid in the flow passage is observable using light. The flow passage device includes a first member ( 2 ) including an observation surface ( 3 ) and an upper inner wall surface ( 4 ), and a second member ( 5 ) including a lower inner wall surface that opposes the upper inner wall surface ( 4 ) and that is part of inner walls of the flow passage. A heating resistor ( 6 ) having a reflective surface that reflects light is provided on the lower inner wall surface. The light reflected by the reflective surface passes through the upper inner wall surface ( 4 ) and the observation surface ( 3 ).

First claim

The invention claimed is:

1. A flow passage device having a flow passage, wherein the flow passage device is able to heat a fluid in the flow passage, and wherein a state of the fluid in the flow passage is observable using light, the flow passage device comprising:
an observation surface used for observing a state in the flow passage;
a first inner wall surface that is part of inner walls of the flow passage; and
a second inner wall surface that opposes the first inner wall surface and that is part of the inner walls of the flow passage,
wherein a heating resistor is provided in the second inner wall surface, wherein the heating resistor has a reflective surface that reflects light, and
wherein the second inner wall surface is, aside from the heating resistor, provided with a reflective member that does not produce heat, wherein the reflective member is provided adjacent to the heating resistor with a gap therebetween.

2. The flow passage device according to claim 1 , wherein the heating resistor has an elongated shape and the reflective member that does not produce heat is provided next to each long side of the heating resistor in such a manner that the reflective member is adjacent to the heating resistor with a gap therebetween.

3. The flow passage device according to claim 1 , wherein the gap is 50 μm or more and 1 mm or less.

4. The flow passage device according to claim 1 ,
wherein a plurality of flow passages are provided, and
wherein each of the plurality of flow passages is disposed parallel to one another.

5. The flow passage device according to claim 1 ,
wherein the heating resistor or each of at least two heating resistors has a transparent insulating layer on the reflective surface thereof, and
wherein the transparent insulating layer serves as a reflection coating.

6. The flow passage device according to claim 1 ,
wherein the reflective surface or each of reflective surfaces is formed of a metal film, and
wherein the metal film includes portions of different thicknesses that are located upstream and downstream with respect to the direction in which the fluid flows in the flow passage or in each of a plurality of flow passages.

7. A testing system comprising:
a receiving table that receives the flow passage device according to claim 1 ;
a light source that emits light toward the flow passage device;
a light detector that detects light from the flow passage device; and
a circuit that supplies a current to the heating resistor or each of at least two heating resistors.

8. The testing system according to claim 7 , further comprising:
a pressure generator that causes the fluid to flow in the flow passage or each of a plurality of flow passages; and
a temperature measuring device that measures a temperature of the fluid in the flow passage or each of the plurality of flow passages in accordance with a resistance of the heating resistor or resistances of the at least two heating resistors.

9. A method of testing a fluid, the method comprising:
preparing the flow passage device according to claim 1 ;
supplying the fluid to the flow passage or to at least one of a plurality of flow passages;
causing the heating resistor or at least one of at least two heating resistors to produce heat to change a temperature of the fluid in the flow passage or in the at least one of the plurality of flow passages; and
detecting an optical state of the fluid in the flow passage or in the at least one of the plurality of flow passages.

10. The method of testing according to claim 9 , the method further comprising measuring a temperature of the fluid in the flow passage or in the at least one of the plurality of flow passages in accordance with resistance of the heating resistor having been heated or at the least one of the at least two heating resistors having been heated.

11. A flow passage device having a flow passage, wherein the flow passage device is able to heat a fluid in the flow passage, and wherein a state of the fluid in the flow passage is observable using light, the flow passage device comprising:
a first member that includes
an observation surface used for observing a state in the flow passage, and
an upper inner wall surface that is part of inner walls of the flow passage; and
a second member that includes
a lower inner wall surface that opposes the upper inner wall surface and that is part of the inner walls of the flow passage,
wherein a heating resistor is provided in the lower inner wall surface of the second member, wherein the heating resistor has a reflective surface that reflects light,
wherein the light reflected by the reflective surface passes through the upper inner wall surface and the observation surface, and
wherein the lower inner wall surface is, aside from the heating resistor, provided with a reflective member that does not produce heat, wherein the reflective member is provided adjacent to the heating resistor with a gap therebetween.

12. The flow passage device according to claim 11 ,
wherein the second member has a depression having a curved surface formed using isotropic etching, and
wherein the depression is covered by a film having the reflective surface.

Assignees

• Ogusu Makoto
• Canon Kk

Inventors

• Ogusu Makoto

Classifications

• B01L7/52 Primary

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• G01N2035/00158

  Elements containing microarrays, i.e. "biochip" · CPC title