Process for fermenting sugars containing oligomeric saccharides

Patent metadata

Field	Value
Publication number	US-9328364-B2
Application number	US-54721105-A
Country	US
Kind code	B2
Filing date	Mar 16, 2005
Priority date	Mar 31, 2004
Publication date	May 3, 2016
Grant date	May 3, 2016

Abstract

Sugar mixtures containing nonfermentable oligomers are fermented in the presence of certain enzymes that depolymerize the oligomers simultaneously with the fermentation process.

First claim

The invention claimed is:

1. A process for fermenting a starch hydrolysate containing 80-98 weight percent of glucose and 1-9 weight percent of nonfermentable glucose oligomers having a degree of polymerization from 2 to 10, which nonfermentable glucose oligomers include isomaltose, comprising forming a fermentation broth containing the starch hydrolysate and having a glucose concentration of at least 50 g/L, fermenting the starch hydrolysate in the presence of a microorganism that ferments the glucose to a desired fermentation product and in the absence of an enzyme that is capable of depolymerizing the nonfermentable glucose oligomer(s) until the fermentation broth contains 26 g/L or less of glucose and from about 1 to about 10 g/L of one or more nonfermentable glucose oligomers, then adding 5-10,000 μL of trans-glucosidase per liter of fermentation broth, and then subjecting the fermentation broth to fermentation conditions in the presence of (A) a microorganism that ferments glucose but does not ferment said nonfermentable glucose oligomers and (B) said trans-glucosidase, under conditions such that the depolymerization of the isomaltose and the fermentation of the glucose occur simultaneously.

2. The process of claim 1 wherein the microorganism ferments the glucose to lactic acid.

3. The process of claim 1 , wherein the glucose oligomers have a degree of polymerization of from 2 to 5.

4. The process of claim 3 wherein the microorganism ferments the glucose to lactic acid.

Assignees

• Beacom Daniel R
• Kolstad Jeffrey J
• Reeder David H
• Rush Brian J
• Cargill Inc

Inventors

• Beacom Daniel R
• Kolstad Jeffrey J
• Reeder David H
• Rush Brian J

Classifications

• C12P19/20

  produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose · CPC title

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P19/16

  produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin (non-biological hydrolysis of starch C08B30/00) · CPC title

• C12P7/56 Primary

  Lactic acid · CPC title