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US-2024352437-A1 · Oct 24, 2024 · US
Kit for identifying bacteria from the Bacillus cereus group
US9322048B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9322048-B2 |
| Application number | US-201414494847-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 24, 2014 |
| Priority date | Sep 18, 2009 |
| Publication date | Apr 26, 2016 |
| Grant date | Apr 26, 2016 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of the Bacillus cereus group comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.
First claim
Opening claim text (preview).
The invention claimed is: 1. A diagnostic kit for identifying bacteria of the Bacillus cereus group comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, said medium comprising at least one inhibitor of Gram-negative bacteria and a first substrate, which first substrate is a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate, wherein the reaction medium does not contain an inhibitor of Gram-positive bacteria. 2. The diagnostic kit as claimed in claim 1 , also comprising a container, selected from the group consisting of microplates, microwells, microtubes, capillaries and multiwell cards. 3. The diagnostic kit as claimed in claim 1 , wherein the fluorescent PC-PLC substrate corresponds to 4 MU-CP (4-methylumbelliferyl choline phosphate). 4. The diagnostic kit as claimed in claim 1 , wherein the reaction medium also comprises at least one second substrate, which is chromogenic or fluorescent. 5. The diagnostic kit as claimed in claim 4 , in which the second substrate is a phosphatidylinositol-specific phospholipase C (PI-PLC) substrate which makes it possible to distinguish Bacillus anthracis from Bacillus cereus, Bacillus thuringiensis, Bacillus weihenstephanensis, Bacillus mycoides and Bacillus pseudomycoides.
Assignees
Inventors
Classifications
- C12Q1/44Primary
involving esterase · CPC title
from Bacillus (G) · CPC title
Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title
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- What does patent US9322048B2 cover?
- The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of the Bacillus cereus group comprising a container of a selective or nonselective reaction me…
- Who is the assignee on this patent?
- Cellier Marie, Mills John, Mosticone David, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/44. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 26 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).