Brassica plants resistant to disease

Patent metadata

Field	Value
Publication number	US-9320211-B2
Application number	US-201013497746-A
Country	US
Kind code	B2
Filing date	Sep 19, 2010
Priority date	Sep 22, 2009
Publication date	Apr 26, 2016
Grant date	Apr 26, 2016

Abstract

The present invention relates to novel Brassica oleracea plants resistant to Albugo candida and the seeds of said plants. The invention also relates to methods of making such plants and for producing seeds thereof. The invention further relates to molecular markers and use thereof in marker assisted breeding and for identifying Albugo candida resistance trait in Brassica oleracea plants.

First claim

What is claimed is:

1. Cultivated Brassica oleracea plant resistant to Albugo candida , comprising a resistance locus, such resistance locus being present in Brassica oleracea L var gemmifera UK 925, seed of which is deposited under Deposit Number NCIMB 41654, and wherein said resistance locus is a monogenic and semi-dominant resistance locus located on chromosome 2, and wherein the Albugo candida resistance locus is genetically linked to at least one marker locus, which co-segregates with Albugo candida resistance trait and comprises a marker that can be identified in a PCR reaction by amplification of a DNA fragment with a pair of PCR oligonucleotide primers represented by a forward primer of SEQ ID NO: 1 and a reverse primer of SEQ ID NO: 2.

2. Cultivated Brassica oleracea plant resistant to Albugo candida according to claim 1 , wherein the resistance locus is a qualitative Albugo candida resistance locus.

3. Cultivated Brassica oleracea plant according to claim 1 wherein the primer pair amplifies a DNA fragment that comprises the at least one marker locus which co-segregates with the Albugo candida resistance locus.

4. Cultivated Brassica oleracea plant according to claim 3 wherein primer pair amplifies a DNA fragment comprising at least one SNP within the at least one marker locus which co-segregates with the Albugo candida resistance locus.

5. Cultivated Brassica oleracea plant according to claim 4 wherein the at least one SNP is selected from the group consisting of:
i. a SNP A represented by a T to C nucleotide exchange at position 134 in the PCR amplified product
ii. a SNP B represented by a C to T nucleotide exchange at position 108 in the PCR amplified product; and
iii. a SNP C represented by a T to C nucleotide exchange at position 366 in the PCR amplified product.

6. Cultivated Brassica oleracea plant according to claim 5 wherein the SNP A can be identified with a pair of PCR oligonucleotide primers represented by a forward primer of SEQ ID NO: 3 and a reverse primer of SEQ ID NO: 4 and DNA probe of SEQ ID NO: 5 defining the resistant allele.

7. Seed of cultivated Brassica oleracea plant according to claim 1 comprising the resistance locus contributing to resistance to Albugo candida.

8. Method for producing a cultivated Brassica oleracea plant, exhibiting resistance to Albugo candida , comprising the steps of:
a. crossing the plant of claim 1 with a cultivated Brassica oleracea plant, which is susceptible to Albugo candida or exhibits a low level of resistance against Albugo candida , and
b. selecting progeny from said cross which exhibits Albugo candida resistance and demonstrates association with said at least one marker locus.

9. Method according to claim 8 , wherein the primer pair amplifies a DNA fragment comprising at least one SNP within the at least one marker locus which co-segregates with the Albugo candida resistance locus.

10. Method according to claim 9 , wherein the at least one SNP is selected from the group consisting of:
i. a SNP A represented by a T to C nucleotide exchange at position 134 in the PCR amplified product
ii. a SNP B represented by a C to T nucleotide exchange at position 108 in the PCR amplified product; and
iii. a SNP C represented by a T to C nucleotide exchange at position 366 in the PCR amplified product.

11. Method for obtaining cultivated Brassica oleracea edible parts resistant to Albugo candida comprising the steps of
i. sowing a seed of cultivated Brassica oleracea according to claim 7 ,
ii. growing said plant in order to produce edible parts and harvesting the said edible parts produced by said plant.

12. Method of protecting a field of cultivated Brassica oleracea plants against infection by Albugo candida , wherein said method is characterized by planting a seed according to claim 7 .

13. Method of producing hybrid seeds of a cultivated Brassica oleracea resistant to Albugo candida comprising the steps of:
i. planting a female Brassica oleracea plant, and the male plant according to claim 1 ,
ii. effecting cross pollination between both parents,
iii. growing the plant till seed setting,
iv. collecting seeds, and
v. obtaining the hybrid seeds.

Assignees

• Linders Enrico Gerardus Albertus
• Wijnker Thaam
• Briggs William
• Van Der Ploeg Sjaak
• Syngenta Participations Ag

Inventors

• Linders Enrico Gerardus Albertus
• Wijnker Thaam
• Briggs William
• Van Der Ploeg Sjaak

Classifications

• C12Q2600/172

  Haplotypes · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• A01H5/00 Primary

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title