Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
US-9222112-B2 · Dec 29, 2015 · US
Fatty acid desaturase used in the biosynthesis of long chain omega-3 polyunsaturated fatty acids
US9315836B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9315836-B2 |
| Application number | US-201414324099-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 4, 2014 |
| Priority date | Oct 6, 2012 |
| Publication date | Apr 19, 2016 |
| Grant date | Apr 19, 2016 |
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- Title
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Abstract
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The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ω3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.
First claim
Opening claim text (preview).
What is claimed is: 1. A process for producing a purified omega-3 desaturase, comprising: (a) constructing a pPinkα-HC vector containing cDNA molecule having the nucleotide sequence shown as SEQ ID NO:1; (b) transforming a suitable host cell with said vector of Step (a), wherein said host cell is a yeast cell; (c) culturing said host cell under conditions which allow the expression of said omega-3 desaturase by said host cell; and (d) isolating said omega-3 desaturase from the culture media of Step (c); wherein said purified omega-3 desaturase is produced. 2. The process of claim 1 , said yeast cell is Pichia pastoris. 3. The process of claim 2 , said Pichia pastoris is selected from a group of hosts consisting of PichiaPink strain 1, PichiaPink strain 2, PichiaPink strain 3, and PichiaPink strain 4. 4. The process of claim 3 , said omega-3 desaturase has the amino acid sequence shown as SEQ ID NO:2.
Assignees
Inventors
Classifications
Miscellaneous (1.14.99) · CPC title
- C12P7/6427Primary
Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone · CPC title
using catalysts, e.g. selective catalysts · CPC title
Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase · CPC title
acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title
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- What does patent US9315836B2 cover?
- The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ω3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.
- Who is the assignee on this patent?
- Univ Jiangnan
- What technology area does this patent fall under?
- Primary CPC classification C12P7/6427. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 19 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).