Lysophospholipid acyltransferase

Patent metadata

Field	Value
Publication number	US-9315835-B2
Application number	US-201414298236-A
Country	US
Kind code	B2
Filing date	Jun 6, 2014
Priority date	Mar 26, 2009
Publication date	Apr 19, 2016
Grant date	Apr 19, 2016

Abstract

The present invention provides novel lysophospholipid acyltransferases. The object of the present invention is attained by the nucleotide sequences of SEQ ID NOs: 1 and 6 and the amino acid sequences of SEQ ID NOs: 2 and 7 of the present invention.

First claim

What is claimed is:

1. A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:
(a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO: 2, and having lysophospholipid acyltransferase activity;
(b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to the nucleotide sequence consisting of SEQ ID NO: 1 and that comprises a nucleotide sequence encoding a protein having lysophospholipid acyltransferase activity;
(c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO: 1 and encoding a protein having lysophospholipid acyltransferase activity;
(d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO: 2 and having lysophospholipid acyltransferase activity; and
(e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO: 2 and that comprises a nucleotide sequence encoding a protein having lysophospholipid acyltransferase activity.

2. The cDNA or recombinant vector of claim 1 , which is (a), (c), or (d) below:
(a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of 1-25 amino acids in the amino acid sequence shown in SEQ ID NO: 2, and having lysophospholipid acyltransferase activity;
(c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 95% or more with the nucleotide sequence consisting of SEQ ID NO: 1 and encoding a protein having lysophospholipid acyltransferase activity; and
(d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 95% or more with the amino acid sequence consisting of SEQ ID NO: 2 and having lysophospholipid acyltransferase activity.

3. A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:
(a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO: 2, and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
(b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to the nucleotide sequence consisting of SEQ ID NO: 1 and that comprises a nucleotide sequence encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
(c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO: 1 and encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
(d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO: 2 and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector; and
(e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO: 2 and that comprises a nucleotide sequence encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector.

4. The cDNA or recombinant vector of claim 3 , which is (a), (c), or (d) below:
(a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of 1-25 amino acids in the amino acid sequence shown in SEQ ID NO: 2, and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
(c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 95% or more with the nucleotide sequence consisting of SEQ ID NO: 1 and encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector; and
(d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 95% or more with the amino acid sequence consisting of SEQ ID NO: 2 and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector.

5. The cDNA or recombinant vector of claim 1 wherein the encoded protein belongs to the membrane-bound O-acyltransferase family.

6. A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(d) below:
(a) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO: 1;
(b) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO: 2;
(c) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO: 4; and
(d) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO: 5.

Assignees

• Suntory Holdings Ltd

Inventors

• Ochiai Misa

Classifications

• A23L1/3008

  Human Necessities · mapped topic

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12P7/6427 Primary

  Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone · CPC title

• A23D9/00

  Other edible oils or fats, e.g. shortenings or cooking oils (preservation of other edible oils A23B20/30) · CPC title

• C12N15/80

  for fungi · CPC title