Sugar chain array

Patent metadata

Field	Value
Publication number	US-9310358-B2
Application number	US-201113812787-A
Country	US
Kind code	B2
Filing date	Jul 29, 2011
Priority date	Jul 30, 2010
Publication date	Apr 12, 2016
Grant date	Apr 12, 2016

Abstract

A sugar chain array containing a sugar chain immobilized thereon for detecting binding between an analyte and a sugar chain, and a sugar chain array having the sugar chain immobilized thereon that is capable of inhibiting non-specific adsorption and binding of an analyte without having to coat the array with an adsorption inhibitor. A specific sugar chain is immobilized on the array, and is useful for detecting binding between the sugar chain and an analyte, such as a pathogen of an infectious disease or an excretion thereof. In addition, in the array, when a base material is coated with a polymeric compound having a unit having a primary amino group, a unit for maintaining hydrophilicity, and a unit having a hydrophobic group, the sugar chain is immobilized efficiently and non-specific adsorption and binding of the analyte can be effectively inhibited.

First claim

The invention claimed is:

1. A sugar chain array, comprising: a base material comprising a cyclic polyolefin polymer; and at least one sugar chain selected from the group consisting of sugar chains from Tables 1, 2, 3, 4, 5 and 6:
{Table 1}
{Table 2}
{Table 3}
{Table 4}
{Table 5}
{Table 6};
wherein the sugar chain is immobilized on the base material, and the base material comprises a coating comprising a polymeric compound having formula [1]:

where R1, R2, and R3 are each independently a hydrogen atom or methyl group, R4 is a cyclohexyl group, X is an alkyleneoxy group comprising 1 to 10 carbon atoms, p is an integer from 1 to 20 and in the case that p is an integer of 2 to 20, the repeating X may be the same or different, Y is a spacer comprising an alkylene glycol residue, Z is an oxygen atom or NH, and l, m and n are each independently a natural number, wherein immobilization of the sugar chain occurs via Schiff base formation between the primary amine group of the polymeric compound and an aldehyde or ketone group of the sugar chain.

2. The sugar chain array of claim 1 ,
wherein the sugar chain is immobilized on the base material by bonding the primary amino group of the polymeric compound to a reducing group on the end of the sugar chain.

3. The sugar chain array of claim 1 , wherein, in formula [1], Y has formula [2]:

wherein, q is an integer from 1 to 20; or

Y has formula [3]:

wherein r is an integer from 1 to 20.

4. The sugar chain array of claim 1 , wherein the primary amino group in the polymeric compound is an oxylamino group.

5. The sugar chain array of claim 1 , wherein the unit having the primary amino group in the polymeric compound is included in an amount of from 20 mol % to 40 mol % based on all units of the polymeric compound.

6. The sugar chain array of claim 1 , wherein, in formula [1], X is an ethyleneoxy group.

7. The sugar chain array of claim 1 , wherein the polymeric compound comprises a (meth)acrylic backbone.

8. The sugar chain array of claim 1 , wherein the unit having a phosphorylcholine group in the polymeric compound is included in an amount of from 5 mol % to 98 mol % based on all units of the polymeric compound.

9. The sugar chain array of claim 1 , wherein the unit having a phosphorylcholine group in the polymeric compound is included in an amount of from 10 mol % to 80 mol % based on all units of the polymeric compound.

10. The sugar chain array of claim 1 , wherein the unit having R4 in the polymeric compound is included in an amount of from 10 mol % to 90 mol % based on all units of the polymeric compound.

11. The sugar chain array of claim 1 , wherein the unit having R4 in the polymeric compound is included in an amount of from 10 mol % to 80 mol % based on all units of the polymeric compound.

12. The sugar chain array of claim 1 , wherein the unit having R4 in the polymeric compound is included in an amount of from 20 mol % to 80 mol % based on all units of the polymeric compound.

13. The sugar chain array of claim 1 , wherein the primary amino group in the polymeric compound is a hydrazide group.

Assignees

• Nishimura Shin-Ichiro
• Matsushita Takahiko
• Shimaoka Hideyuki
• Takada Wataru
• Igarashi Kohta
• Abe Midori
• Abe Hiroki
• Fukushima Masao
• Sumitomo Bakelite Co
• Univ Hokkaido Nat Univ Corp

Inventors

• Nishimura Shin-Ichiro
• Matsushita Takahiko
• Shimaoka Hideyuki
• Takada Wataru
• Igarashi Kohta
• Abe Midori
• Abe Hiroki
• Fukushima Masao

Classifications

• G01N33/66 Primary

  involving blood sugars, e.g. galactose · CPC title

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• G01N33/5308 Primary

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title

• G01N33/569

  for microorganisms, e.g. protozoa, bacteria, viruses · CPC title

• G01N2333/42

  Lectins, e.g. concanavalin, phytohaemagglutinin · CPC title