Method for extracting components from a yeast cell culture

Patent metadata

Field	Value
Publication number	US-9309549-B2
Application number	US-74553707-A
Country	US
Kind code	B2
Filing date	May 8, 2007
Priority date	May 10, 2006
Publication date	Apr 12, 2016
Grant date	Apr 12, 2016

Abstract

The present invention relates to a method for extracting components from yeast cells with a purified phospholipase.

First claim

The invention claimed is:

1. A method for producing a yeast extract, the method comprising:
i) treating yeast cells with a purified phospholipase A1 in an amount of between 0.001 and 1 mg enzyme protein/g dry matter;
ii) subjecting the treated yeast cells to protein hydrolysis comprising autolysis and comprising adding one or more proteolytic enzymes to the yeast cells; and
iii) separating a yeast extract from the treated yeast cells;
wherein step ii) is conducted before, after or during step i).

2. The method of claim 1 , wherein phospholipase A1 is added in an amount of between 0.005 and 0.1 mg enzyme protein/g dry matter.

3. The method of claim 1 , wherein protein hydrolysis further comprises plasmolysis.

4. The method of claim 1 , further comprising disrupting the yeast cells before the separation.

5. The method of claim 4 , comprising homogenisation and/or addition of one or more components that facilitate cell disruption.

6. The method of claim 4 , comprising adding sodium chloride, ethyl acetate or isopropanol.

7. The method of claim 1 , further comprising treating yeast cells with a phospholipase A2.

8. The method of claim 1 , further comprising treating yeast cells with a phospholipase B.

9. The method of claim 1 , further comprising treating yeast cells with a phospholipase C.

10. The method of claim 1 , further comprising treating yeast cells with a phospholipase D.

Assignees

• Kalum Lisbeth
• Nielsen Per Munk
• Ernst Steffen
• Novozymes As

Inventors

• Kalum Lisbeth
• Nielsen Per Munk
• Ernst Steffen

Classifications

• A23L33/145

  Extracts · CPC title

• C12N1/063

  of yeast · CPC title

• C12P21/06 Primary

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• C12N9/18

  Carboxylic ester hydrolases {(3.1.1)} · CPC title

• A23L1/3018

  Human Necessities · mapped topic