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Phage displaying system expressing single chain antibody
US9309522B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9309522-B2 |
| Application number | US-201313897850-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 20, 2013 |
| Priority date | Aug 11, 2009 |
| Publication date | Apr 12, 2016 |
| Grant date | Apr 12, 2016 |
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Abstract
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Disclosed are nucleic acid libraries for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and for facilitating production of a disulfide-stabilized single chain antibody. Also disclosed are host cell libraries and phage libraries including the nucleic acid libraries. Further disclosed are methods for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and methods for producing a disulfide-stabilized single chain antibody and non-fusion form thereof.
First claim
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We claim: 1. An isolated nucleic acid, comprising a first nucleotide sequence encoding a signal peptide, and a second nucleotide sequence encoding a single chain antibody capable of forming an interface disulfide bond, the second nucleotide sequence being located 3′ downstream to the first nucleotide, wherein the signal peptide has the amino acid sequence of VKKLLX 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 AAQPAMAHHHHHHGH (SEQ ID NO:596), in which X 1 is A, C, F, G, I, L, M, P,…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N15/73Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9309522B2 cover?
- Disclosed are nucleic acid libraries for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and for facilitating production of a disulfide-stabilized single chain antibody. Also disclosed are host cell libraries and phage libraries including the nucleic acid libraries. Further disclosed are methods for identifying a signal peptide that facili…
- Who is the assignee on this patent?
- Academia Sinica
- What technology area does this patent fall under?
- Primary CPC classification C12N15/73. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 12 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).