Phased small RNAs

Patent metadata

Field	Value
Publication number	US-9309512-B2
Application number	US-201313775477-A
Country	US
Kind code	B2
Filing date	Feb 25, 2013
Priority date	Aug 31, 2006
Publication date	Apr 12, 2016
Grant date	Apr 12, 2016

Abstract

This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.

First claim

What is claimed is:

1. A recombinant DNA construct comprising a promoter operably linked to DNA that transcribes to RNA comprising:
(a) at least one exogenous recognition site recognizable by a phased small RNA expressed in a specific plant cell of a plant, and
(b) target RNA to be suppressed in said specific plant cell,
wherein said target RNA is to be expressed in cells of said plant other than said specific plant cell,
wherein said phased small RNA has a nucleotide sequence that maps to the plus strand of an endogenous phased small RNA locus,
wherein said phased small RNA locus contains at least three contiguous phased small RNAs,
wherein said at least three contiguous phased small RNAs target multiple genes or different segments of a single gene, and wherein said phased small RNA locus is cleaved in phase by a plant DCL4 ribonuclease.

2. The recombinant DNA construct of claim 1 , wherein said at least one exogenous recognition site is located within at least one of:
(a) the 5′ untranslated region of said target RNA;
(b) the 3′ untranslated region of said target RNA; and
(c) said target RNA.

Assignees

• Monsanto Technology Llc

Inventors

• Allen Edwards
• Guo Liang
• Heisel Sara E
• Ivashuta Sergey I
• Zhang Yuanji

Classifications

• C12N15/8218 Primary

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8266

  Abscission; Dehiscence; Senescence · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N15/827

  Flower development or morphology, e.g. flowering promoting factor [FPF] · CPC title

• C12N15/8245

  involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title