Polypeptides having glucoamylase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9309505-B2
Application number	US-201013261652-A
Country	US
Kind code	B2
Filing date	Nov 30, 2010
Priority date	Nov 8, 2010
Publication date	Apr 12, 2016
Grant date	Apr 12, 2016

Abstract

The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A process of producing a fermentation product from starch-containing material comprising the steps of:
(a) liquefying starch-containing material;
(b) saccharifying the liquefied material; and
(c) fermenting with a fermenting organism;

wherein step (b) is carried out using at least a
polypeptide having glucoamylase activity selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2; and
(b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

2. A process of producing a fermentation product from starch-containing material, comprising the steps of:
(a) saccharifying starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material; and
(b) fermenting with a fermenting organism,

wherein step (a) is carried out using at least
a polypeptide having glucoamylase activity selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2; and
(b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

3. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

4. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

5. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.

6. The process of claim 1 , wherein the polypeptide having glucoamylase activity consists of the mature polypeptide of SEQ ID NO: 2.

7. The process of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

8. The process of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 98% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

9. The process of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 99% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

10. The process of claim 1 , wherein the polypeptide consists of the amino acids 21 to 472 of SEQ ID NO: 2.

11. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

12. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

13. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.

14. The process of claim 2 , wherein the polypeptide having glucoamylase activity consists of the mature polypeptide of SEQ ID NO: 2.

15. The process of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

16. The process of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 98% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

17. The process of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 99% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO: 2.

18. The process of claim 2 , wherein the polypeptide consists of the amino acids 21 to 472 of SEQ ID NO: 2.

19. The process of claim 1 , wherein the mature polypeptide is amino acids 20 to 573 of SEQ ID NO: 2.

20. The process of claim 2 , wherein the mature polypeptide is amino acids 20 to 573 of SEQ ID NO: 2.

21. A method of producing a polypeptide comprising: (a) cultivating a host cell comprising a heterologous nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide, wherein the polypeptide has glucoamylase activity and comprises an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

Assignees

• Landvik Sara
• Ayabe Keiichi
• Coward-Kelly Guillermo
• Novozymes North America Inc
• Novozymes As

Inventors

• Landvik Sara
• Ayabe Keiichi
• Coward-Kelly Guillermo

Classifications

• A23L1/095

  Human Necessities · mapped topic

• C12N15/8245

  involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C07K2319/20

  containing a tag with affinity for a non-protein ligand · CPC title

• A23L1/034

  Human Necessities · mapped topic