Electron conducting crosslinked polyaniline-based redox hydrogel, and method of making

Patent metadata

Field	Value
Publication number	US-9303279-B2
Application number	US-201414248005-A
Country	US
Kind code	B2
Filing date	Apr 8, 2014
Priority date	May 3, 2007
Publication date	Apr 5, 2016
Grant date	Apr 5, 2016

Abstract

A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol)diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.

First claim

What is claimed is:

1. An electrode, comprising:
a conductive substrate and a crosslinked polymeric matrix deposited thereon comprising a polyaniline crosslinked with a polymer acid and a redox enzyme through a crosslinker.

2. The electrode according to claim 1 , wherein the polyaniline is a ring-substituted polyaniline.

3. The electrode according to claim 2 , wherein the ring-substituted polyaniline is a compound selected from the group consisting of poly-meta-toluidine, poly-ortho-toluidine, poly-ortho-fluoroaniline, poly-ortho-methoxyaniline and poly-ortho,ortho′-dimethylaniline.

4. The electrode according to claim 1 , wherein the polymer acid is poly(2-acrylamido-2-methyl-propane sulfonic acid) (PAAMSA).

5. The electrode according to claim 4 , wherein the polyaniline and PAAMSA are present in a molar ratio of from about 1:0.7 to about 1:0.99.

6. The electrode according to claim 1 , wherein the crosslinker is a water soluble crosslinker.

7. The electrode according to claim 6 , wherein the water soluble crosslinker comprises poly(ethylene glycol) diglycidyl ether.

8. The electrode according to claim 7 , wherein the conductive substrate comprises carbon.

9. The electrode according to claim 8 , wherein the conductive substrate comprises a corrosion resistant conductor surface.

10. The electrode according to claim 6 , wherein the water-soluble crosslinker is present in an amount of from about 2 weight % to about 30 weight %, based upon the dry weight of the polymer matrix.

11. The electrode according to claim 1 , wherein the crosslinked polymeric matrix is a hydrogel.

12. The electrode according to claim 11 , wherein the hydrogel further comprises water.

13. The electrode according to claim 1 , wherein the redox enzyme is selected from the group consisting of a flavoenzyme, a heme enzyme, and a PQQ enzyme.

14. The electrode according to claim 1 , wherein the redox enzyme is selected from the group consisting of glucose oxidase, glucose dehydrogenase, and peroxidase.

15. The electrode sensor according to claim 1 , wherein the redox enzyme of the crosslinked polymer matrix has an apparent Michaelis-constant obtained from an Edi-Hofstee plot of greater than 10 mM glucose.

16. The electrode according to claim 1 , wherein the crosslinked polymer matrix comprises two enzymes, wherein a first enzyme is an enzyme which catalyzes a reaction to form a substrate for the redox enzyme.

17. The electrode according to claim 1 , wherein the redox enzyme is present in an amount of from greater than 0 weight % to about 60 weight %, based upon the dry weight of the polymer matrix.

Assignees

• Abbott Diabetes Care Inc

Inventors

• Heller Adam
• Feldman Benjamin J
• Mano Nicolas
• Loo Yueh-Lin

Classifications

• G01N27/3271 Primary

  Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood (amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001) · CPC title

• C12Q1/001 Primary

  Enzyme electrodes · CPC title

• G01N27/327

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• C12Q1/005

  involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence) · CPC title

• C12Q1/006

  for glucose · CPC title