Inkjet printing of tissues and cells

Patent metadata

Field	Value
Publication number	US-9301925-B2
Application number	US-201514644678-A
Country	US
Kind code	B2
Filing date	Mar 11, 2015
Priority date	Feb 14, 2008
Publication date	Apr 5, 2016
Grant date	Apr 5, 2016

Abstract

Provided herein is an apparatus for printing cells which includes an electrospinning device and an inkjet printing device operatively associated therewith. Methods of making a biodegradable scaffold having cells seeded therein are also provided. Methods of forming microparticles containing one or more cells encapsulated by a substrate are also provided, as are methods of forming an array of said microparticles.

First claim

That which is claimed is:

1. A method of forming microparticles comprising one or more cells encapsulated by a substrate, said method comprising printing a composition comprising said cells and said substrate into a solution that promotes gelling of the substrate,
wherein said substrate comprises alginate, and wherein said solution that promotes gelling of the substrate comprises divalent cations,
to thereby form said microparticles,
wherein said microparticles comprise a membrane of said substrate encapsulating 10 cells or less in at least 50% of the printed cells.

2. The method of claim 1 , wherein said divalent cations comprise Mg 2+ , Ca 2+ , Sr 2+ and/or Ba 2+ .

3. The method of claim 1 , wherein said microparticles have an average diameter of from 5 to 50 micrometers.

4. The method of claim 1 , wherein greater than 50% of said microparticles formed contain a single cell encapsulated by said substrate.

5. The method of claim 1 , wherein said cells are selected from the group consisting of pancreas cells, kidney cells, liver cells and nerve cells.

6. The method of claim 1 , wherein said cells are pancreatic islet cells.

7. The method of claim 6 , wherein said pancreatic islet cells secrete insulin.

8. The method of claim 1 , further comprising the steps of:
providing an inkjet printing device, said device comprising at least one inkjet printer cartridge; and
loading a composition comprising said cells and said substrate into said printer cartridge, prior to said printing step.

9. The method of claim 8 , wherein said cells are included at a concentration of between 10 5 and 10 7 cells per milliliter of said composition.

10. The method of claim 8 , wherein said substrate is alginate and said composition comprises between 0.5 and 2% w/v of said substrate.

11. The method of claim 1 , wherein more than 80% of said cells by number are viable 24 hours after printing.

12. A method of forming an array of microparticles comprising one or more cells encapsulated by a substrate comprising the steps of:
providing an inkjet printing device, said device comprising at least one inkjet printer cartridge;
loading a composition comprising said cells and said substrate into said printer cartridge; wherein said substrate comprises alginate; and
printing said composition in an organized pattern into a solution that promotes gelling of the substrate, wherein said solution comprises divalent cations, to thereby form said array of microparticles,
wherein said microparticles comprise a membrane of said substrate encapsulating 10 cells or less in at least 50% of the printed cells.

13. The method of claim 12 , wherein said microparticles have an average diameter of from 5 to 50 micrometers.

14. The method of claim 12 , wherein greater than 50% of said microparticles contain a single cell encapsulated by said substrate.

15. The method of claim 12 , wherein said cells are selected from the group consisting of pancreas cells, kidney cells, liver cells and nerve cells.

16. The method of claim 12 , wherein said cells are pancreatic islet cells.

17. The method of claim 12 , wherein said cells secrete insulin.

18. The method of claim 12 , wherein said divalent cations comprise Mg 2+ , Ca 2+ , Sr 2+ and/or Ba 2+ .

19. The method of claim 12 , wherein said cells of said loading step are included at a concentration of between 10 5 and 10 7 cells per milliliter of said composition.

20. The method of claim 12 , wherein said substrate is alginate and said composition of said loading step comprises between 0.5 and 2% w/v alginate.

21. The method of claim 12 , wherein more than 80% of said cells by number are viable 24 hours after printing.

Assignees

• Univ Wake Forest Health Sciences

Inventors

• Xu Tao
• Yoo James J
• Atala Anthony

Classifications

• A61L27/50

  Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} · CPC title

• A61K9/5089 Primary

  Processes · CPC title

• A61F2/062

  Apparatus for the production of blood vessels made from natural tissue or with layers of living cells · CPC title

• D01F6/625

  derived from hydroxy-carboxylic acids, e.g. lactones · CPC title

• B41J3/407

  for marking on special material · CPC title