Directed surface functionalization on selected surface areas of topographical features with nanometer resolution

Patent metadata

Field	Value
Publication number	US-9297062-B2
Application number	US-201314071007-A
Country	US
Kind code	B2
Filing date	Nov 4, 2013
Priority date	Oct 15, 2013
Publication date	Mar 29, 2016
Grant date	Mar 29, 2016

Abstract

A method for making a single molecule receptor in a nanopore structure includes depositing a material by a physical vapor deposition (PVD) technique onto a selected interior surface of a nanochannel and functionalizing a surface of the material with a chemical compound having at least two functional groups. The material forms a patch having a diameter of about 3 to about 10,000 nanometers (nm). Also disclosed are embodiments of a nanopore structure including a single molecule receptor.

First claim

What is claimed is:

1. A method for making a single molecule receptor in a nanopore structure, the method comprising:
depositing a material by a physical vapor deposition (PVD) technique onto a selected interior surface of a nanochannel, the material forming a raised patch having a diameter of about 1 to about 100 nanometers (nm) and extending from the selected area of the interior surface to the nanochannel's opening; and
functionalizing a surface of the material with a chemical compound having at least two functional groups to form the single molecule receptor;
wherein the single molecule receptor is a binding site with nanometer dimensions that binds to only a single molecule of an analyte in a complex sample.

2. The method of claim 1 , wherein the material a metal, a metal alloy, a semiconductor, an insulator, or any combination thereof.

3. The method of claim 2 , wherein the material is aluminum, platinum, tungsten, gold, silver, nickel, palladium, carbon, cobalt, or any combination thereof.

4. The method of claim 1 , wherein the PVD technique is pulsed laser deposition/ablation (PLD), sputtering, electron-beam deposition, pulsed electron deposition (PED), focused ion beam (FIB) deposition, or any combination thereof.

5. The method of claim 1 , wherein one of the at least two functional groups is a group that forms a contact product with the material and is an acyl halide group, an amine group, an amide group, an alcohol group, a carboxylate thiol group, a nitrile group, a phosphate group, a phosphine group, silane group, a sulfate group, a sulfide group, a sulfite group, thiol group, a thiolate group, or any combination thereof.

6. A method for making a single molecule receptor in a nanopore structure, the method comprising:
depositing a material by a PVD technique onto a selected interior surface of a nanochannel, the material forming a raised patch having a diameter of about 1 to about 100 nm and a thickness of about 0.5 to about 10 nm and extending from the selected area of the interior surface to the nanochannel's opening; and
functionalizing a surface of the material with a chemical compound having at least two functional groups to form the single molecule receptor;
wherein the single molecule receptor is a binding site that binds to a single molecule of an analyte in a single binding event.

Assignees

• IBM
• Globalfoundries Inc

Inventors

• Baldauf Julia
• Harrer Stefan
• Schieber Christine

Classifications

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• C23C14/34

  Sputtering · CPC title

• C23C14/221

  Ion beam deposition (C23C14/46, C23C14/48 take precedence) · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title