Centrifugal filter

Patent metadata

Field	Value
Publication number	US-9295947-B2
Application number	US-201414190560-A
Country	US
Kind code	B2
Filing date	Feb 26, 2014
Priority date	Sep 24, 2007
Publication date	Mar 29, 2016
Grant date	Mar 29, 2016

Abstract

Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.

First claim

What is claimed is:

1. A centrifugal filtration assembly, comprising:
a filtration device and at least one filtration device holder;
said filtration device comprising a sample reservoir, a pair of spaced membranes each supported by an underdrain support comprising a series of channels, grooves or surface textures to capture filtrate, a plurality of drain holes for draining said filtrate, and a retentate chamber, said filtration device having an external diameter;
said device holder comprising a housing having an open end and an internal diameter;
wherein said filtration device is insertable into said device holder in a spin mode whereby filtrate from said filtration device flows from said drain holes into said device holder housing, and in a reverse spin mode whereby retentate in said retentate chamber flows into said device holder housing, and wherein the extent of the inserting of said filtration device into said device holder is delimited by the relative lengths of said external diameter and internal diameter.

2. The centrifugal filtration assembly of claim 1 , wherein each said membrane is oriented at an angle of greater than about 0° and less than about 5° from the vertical centerline of said filtration device.

3. The centrifugal filtration assembly of claim 1 , wherein each said membrane is oriented at an angle of about 3° from the vertical centerline of said filtration device.

4. The centrifugal filtration assembly of claim 1 , wherein said retentate chamber comprises a dead volume.

Assignees

• Emd Millipore Corp

Inventors

• Bonhomme Louis
• Doyle John
• Greenizen Kurt
• Gagne George
• Devin Sean
• Lombard Gerard
• Smith Vincent

Classifications

• B01L3/5635

  connecting two containers face to face, e.g. comprising a filter · CPC title

• B01D63/08

  Flat membrane modules · CPC title

• B01D2313/08

  Flow guidance means within the module or the apparatus · CPC title

• B01D61/145

  Ultrafiltration · CPC title

• B01D61/18

  Apparatus therefor · CPC title