Clubroot resistance Brassica oleracea plants

Patent metadata

Field	Value
Publication number	US-9295207-B2
Application number	US-201113114176-A
Country	US
Kind code	B2
Filing date	May 24, 2011
Priority date	Jul 26, 2002
Publication date	Mar 29, 2016
Grant date	Mar 29, 2016

Abstract

The present invention discloses B. oleracea plants resistant to clubroot disease. In particular, the plants of the present invention comprise a monogenic dominant resistance to the disease clubroot introgressed from B. rapa . This resistance provides improved resistance to the disease as compared to previously existing resistances in B. oleracea.

First claim

What is claimed is:

1. A method for introgressing a monogenic and dominant resistance to clubroot from B. rapa into B. oleracea comprising the steps of:
a) crossing a diploid B. rapa plant with a diploid B. oleracea plant, wherein said B. rapa plant comprises a monogenic and dominant resistance to clubroot;
b) rescuing embryos resulting from the cross of step a);
c) regenerating a hybrid F1 plant having 19 chromosomes from an embryo of step b);
d) selecting a plant of step c) that is resistant to clubroot, wherein the resistance to clubroot is monogenic and dominant;
e) back-crossing the plant resulting from step d) with a B. oleracea plant;
f) rescuing embryos resulting from the cross of step e);
g) regenerating a plant having from 18 to 28 chromosomes from an embryo of step f);
h) selecting a plant of step g) that is resistant to clubroot, wherein the resistance to clubroot is monogenic and dominant;
i) optionally backcrossing the plant of step h) for one or more generations to a B. oleracea plant to produce progeny plants; and
j) selecting a plant from the later of step h) or step i), wherein said selected plant is a B. oleracea plant having 18 chromosomes and comprising a monogenic and dominant resistance to clubroot, thereby introgressing a monogenic and dominant resistance to clubroot from B. rapa into B. oleracea.

2. The method according to claim 1 , further comprising introgreesing the resistance into an elite B. oleracea inbred.

3. The method according to claim 2 , further comprising crossing said inbred to another B. oleracea inbred to produce a hybrid.

4. The method of claim 1 wherein the B. rapa plant is Chinese cabbage F1 hybrid Parkin.

Assignees

• Linders Enrico Gerardus Albertus
• Tjeertes Peter
• De Haas Johannes Maria
• Huang Cai-Cheng
• Syngenta Participations Ag

Inventors

• Linders Enrico Gerardus Albertus
• Tjeertes Peter
• De Haas Johannes Maria
• Huang Cai-Cheng

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• A01H5/10

  Seeds · CPC title

• A01H1/04 Primary

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title