Biomarkers for determining an allograft tolerant phenotype

Patent metadata

Field	Value
Publication number	US-9290813-B2
Application number	US-201013512539-A
Country	US
Kind code	B2
Filing date	Dec 1, 2010
Priority date	Dec 2, 2009
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

Methods are provided for determining whether a subject has a graft tolerant phenotype. In practicing the subject methods, the expression level of one or more gene in a sample from the subject, e.g., a blood sample, is assayed to obtain a gene expression result, where the gene expression result includes a result for a biomarker of graft tolerance. The obtained gene expression result is then employed to determine whether the subject has a graft tolerant phenotype. Also provided are compositions, systems and kits that find use in practicing the subject methods. The methods and compositions find use in a variety of applications, including the determination of an immunosuppressive therapy regimen.

First claim

What is claimed is:

1. A method of determining whether a subject who has received renal allograft has a graft tolerant or a graft intolerant phenotype comprising:
(a) evaluating the level of expression of at least three genes in a sample from a subject who has received a renal allograft to obtain a gene expression result, wherein said at least three genes are selected from the group consisting of: BNC2, C1QC, CCL4, CYP1B1, FAM110C, GDEP, IGFL2, IGH@, IGHA2, IGHG4, IGJ, KLF6, NXF3, PRAMEF3, RLBP1L1, SHCBP1, SPC25, TFDP3, TNFRSF17, UHRF1 and VN1R2, wherein said evaluating comprises:
contacting said sample with a collection of reagents for assaying for an expression product of each of said at least three genes; and
assessing, using said collection of reagents, the amount of each of said expression products in said sample;
(b) comparing said gene expression result to at least one reference gene expression profile, wherein said reference gene expression profile is selected from: a graft tolerant phenotype gene expression profile and a graft intolerant phenotype gene expression profile; and
(c) determining that said subject has a graft tolerant phenotype based on said comparing when said gene expression result is similar to said graft tolerant phenotype gene expression profile and/or is dissimilar to said graft intolerant phenotype gene expression profile; or determining that said subject has a graft intolerant phenotype based on said comparing when said gene expression result is dissimilar to said graft tolerant phenotype gene expression profile and/or is similar to said graft intolerant phenotype gene expression profile.

2. The method according to claim 1 , wherein said at least three genes comprises: FAM110C, IGHG4 and KLF6.

3. The method according to claim 1 , wherein said sample is a blood sample.

4. The method according to claim 1 , wherein said expression product is selected from one or both of: a nucleic acid transcript and a protein.

5. The method of claim 4 , wherein said expression product is a nucleic acid transcript and said evaluating step comprises performing one or more of the following: a RT-PCR assay, a microarray assay, and a Northern blot.

6. The method according to claim 1 , wherein the expression level of BNC2, C1QC, CCL4, CYP1B1, FAM110C, GDEP, IGFL2, IGH@, IGHA2, IGHG4, IGJ, KLF6, NXF3, PRAMEF3, RLBP1L1, SHCBP1, SPC25, TFDP3, TNFRSF17, UHRF1 and VN1R2 is evaluated.

7. A method of managing immunosuppressive therapy treatment in a subject having an allograft, said method comprising:
(a) determining whether said subject has a graft tolerant phenotype according to claim 1 ; and
(b) managing future immunosuppressive therapy treatment in said subject based on said determining step (a).

8. A system for determining whether a subject who has received a renal allograft has a graft tolerant or a graft intolerant phenotype, said system comprising:
(a) a gene expression evaluation element for evaluating the level of expression of at least three genes in a sample from said subject to obtain a gene expression result, wherein said at least three genes are selected from the group consisting of: BNC2, CCL4, C1QC, CYP1B1, FAM110C, GDEP, IGJ, IGH@, IGHA2, IGHG4, IGFL2, KLF6, NXF3, PRAMEF3, RLBP1L1, SHCBP1, SPC25, TFDP3, TNFRSF17, UHRF1, and VN1R2; and
(b) a phenotype determination element for employing said gene expression element and determining whether said subject has a graft tolerant or a graft intolerant phenotype, the phenotype determination element comprising a reference expression profile for said at least three genes, wherein the phenotype determination element is obtained from a sample from a patient with a graft tolerant phenotype or a graft intolerant phenotype.

9. The system according to claim 8 , wherein said gene expression evaluation element comprises a collection of reagents for assaying a sample for an expression product of each of said at least three genes.

10. The system according to claim 8 , wherein said expression product of said at least three genes are selected from: a nucleic acid transcript and a protein.

11. The method according to claim 1 , wherein said at least three genes comprises: CYP1B1, and BNC2.

12. The method according to claim 1 , wherein said reference gene expression profile is from a subject having induced graft tolerance.

13. The method according to claim 1 , wherein said comparing step comprises at least one of: comparing digital images of the expression profiles and comparing databases of expression data.

14. The method according to claim 3 , wherein said blood sample is a peripheral blood lymphocyte sample.

15. The method according to claim 1 , wherein said assessing is quantitative.

16. A method of determining whether a subject who has received a renal allograft has a graft tolerant or a graft intolerant phenotype comprising:
(a) obtaining a peripheral blood sample comprising a peripheral blood lymphocyte from a subject;
(b) evaluating the level of expression of at least three genes in said peripheral blood sample to obtain a gene expression result, wherein said at least three genes are selected from the group consisting of: BNC2, CCL4, C1QC, CYP1B1, FAM110C, GDEP, IGJ, IGH@, IGHA2, IGHG4, IGFL2, KLF6, NXF3, PRAMEF3, RLBP1L1, SHCBP1, SPC25, TFDP3, TNFRSF17, UHRF1, and VN1R2, and wherein said evaluating comprises:
extracting mRNA from said sample;
contacting said mRNA with a collection of reagents for assaying mRNA from each of said at least three genes;
assessing, using said collection of reagents, the amount of said mRNA from each of said at least three genes in said sample;

(c) comparing said gene expression result to at least one reference gene expression profile, wherein said reference gene expression profile is selected from: a graft tolerant phenotype gene expression profile and a graft intolerant phenotype gene expression profile; and
(d) determining that said subject has a graft tolerant phenotype based on said comparing when said gene expression result is similar to said graft tolerant phenotype gene expression profile and/or is dissimilar to said graft intolerant phenotype gene expression profile; or determining said subject has a graft intolerant phenotype based on said comparing when said gene expression result is dissimilar to said graft tolerant phenotype gene expression profile and/or is similar to said graft intolerant phenotype gene expression profile.

17. A method of treating a transplant recipient, said method comprising:
(a) evaluating whether said transplant recipient has a graft tolerant or a graft intolerant phenotype by using a gene expression result that was previously obtained from a quantitative determination of the nucleic acid expression levels of at least three genes in a sample from said a transplant recipient, wherein said at least at least three genes are selected from the group consisting of: BNC2, CCL4, C1QC, CYP1B1, FAM110C, GDEP, IGJ, IGH@, IGHA2, IGHG4, IGFL2, KLF6, NXF3, PRAMEF3, RLBP1L1, SHCBP1, SPC25, TFDP3, TNFRSF17, UHRF1, and VN1R2;
(b) comparing said previously obtained gene expression result to at least one reference gene expression profile, wherein said reference gene expression profile is selected from: a graft tolerant phenotype gene expression profile and a graft intolerant phenotype gene expression profile;
(c) determining that said transplant recipient has a graft tolerant phenotype based on said comparing when said gene expression result is similar to said graft tolerant phenotype gene expression profile and/or is dissimilar to said graft intolerant phenotype gene express

Assignees

• Sarwal Minnie M
• Li Li
• Strober Samuel
• Univ Leland Stanford Junior

Inventors

• Sarwal Minnie M
• Li Li
• Strober Samuel

Classifications

• C12Q1/6883 Primary

  for diseases caused by alterations of genetic material · CPC title

• C12Q2600/106

  Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism · CPC title

• C12Q2600/158

  Expression markers · CPC title

• G01N2800/245

  Transplantation related diseases, e.g. graft versus host disease · CPC title

• G01N33/6893

  related to diseases not provided for elsewhere · CPC title