Composition containing 2-acyl-lysophosphatidylserine and method for producing the same

Patent metadata

Field	Value
Publication number	US-9290781-B2
Application number	US-201113824518-A
Country	US
Kind code	B2
Filing date	Sep 26, 2011
Priority date	Sep 30, 2010
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

The present invention provides a method for producing a composition containing 2-acyl-lysophosphatidylserine, including (a) a step of obtaining a composition containing phosphatidylserine by allowing phospholipase D to act on a raw material containing phosphatidylcholine in the presence of serine and (b) a step of obtaining a composition containing 2-acyl-lysophosphatidylserine by allowing phospholipase A1 to act on the composition containing phosphatidylserine in the presence of one or more additives selected from the group consisting of the following (I), (II), and (III), in which (I) one or more salts selected from the group consisting of sulfate salts, phosphate salts, nitrate salts, citrate salts, and tartarate salts of monovalent cations, (II) a gum, and (III) an emulsifier.

First claim

The invention claimed is:

1. A method for producing a composition containing 2-acyl-lysophosphatidylserine, the method consisting of:
(a) obtaining a composition containing phosphatidylserine by allowing phospholipase D to act on a raw material containing phosphatidylcholine in the presence of serine to produce phosphatidylserine; and
(b) treating the composition containing phosphatidylserine obtained in (a) with phospholipase A1 in the presence of at least one selected from each of (I), (II), and (III):
(I) one or more salts selected from the group consisting of sulfate salts, phosphate salts, nitrate salts, citrate salts, and tartarate salts of monovalent cations,
(II) a gum selected from the group consisting of gum arabic present in a concentration of 1 to 10%, xanthum gum present in a concentration of 0.1%, tamarind gum present in a concentration of 1 to 3%, tara gum present in a concentration of 0.5% and locust bean gum present in a concentration of 1%, and
(III) an emulsifier
to obtain a composition containing 2-acyl lysophosphatidylserine containing 26% to 51% by mass of a lysophospholipid per solid content.

2. A method for producing a composition containing 2-acyl-lysophosphatidylserine, consisting of:
(c) obtaining a composition containing 2-acyl-lysophosphatidylcholine by allowing phospholipase A1 to act on a raw material containing phosphatidylcholine in the presence of at least one selected from each of (I), (II), and (III):
(I) one or more salts selected from the group consisting of sulfate salts, phosphate salts, nitrate salts, citrate salts, and tartarate salts of monovalent cations,
(II) a gum selected from the group consisting of gum arabic present in a concentration of 1 to 10%, xanthum gum present in a concentration of 0.1%, tamarind gum present in a concentration of 1 to 3%, tara gum present in a concentration of 0.5% and locust bean gum present in a concentration of 1%, and
(III) an emulsifier
to produce 2-acyl-lysophosphatidylcholine; and
(d) treating the composition containing 2-acyl-lysophosphatidylcholine obtained in (c) with phosphaliase D in the presence of serine to produce a composition containing 2-acyl-lysophosphatidylserine containing 26% to 51% by mass of a lysophospholipid per solid content.

3. The producing method according to claim 1 , wherein the treating step (b) is carried out in the presence of at least the one or more salts of monovalent cations, wherein a concentration of the one or more salts of monovalent cations is 0.25 to 2.0 M.

4. The producing method according to claim 2 , wherein the treating step (c) is carried out in the presence of at least the one or more salts of monovalent cations, wherein a concentration of the one or more salts of monovalent cations is 0.25 to 2.0 M.

5. The producing method according to claim 1 , wherein the treating step (b) is carried out in the presence of at least the one or more salts of monovalent cations, and the one or more salts of the cations is ammonium sulfate and/or sodium sulfate.

6. The producing method according to claim 2 , wherein the treating step (c) is carried out in the presence of at least the one or more salts of monovalent cations, and the one or more salts of the cations is ammonium sulfate and/or sodium sulfate.

7. The producing method according to claim 1 , wherein the treating step (b) is carried out in the presence of at least the emulsifier, wherein the emulsifier is one or more selected from the group consisting of sucrose fatty acid ester emulsifiers, polyglycerol fatty acid ester emulsifiers, and polysorbate emulsifiers.

8. The producing method according to claim 2 , wherein the treating step (c) is carried out in the presence of at least the emulsifier, wherein the emulsifier is one or more selected from the group consisting of sucrose fatty acid ester emulsifiers, polyglycerol fatty acid ester emulsifiers, and polysorbate emulsifiers.

9. The producing method according to claim 1 , wherein the raw material containing phosphatidylcholine is derived from marine and/or plant.

10. The producing method according to claim 2 , wherein the raw material containing phosphatidylcholine is derived from marine and/or plant.

11. The producing method according to claim 1 , wherein the raw material containing phosphatidylcholine is derived from marine.

12. The producing method according to claim 2 , wherein the raw material containing phosphatidylcholine is derived from marine.

13. The method according to claim 1 , wherein the percentage of the lysophospholipid in the total phospholipid in the composition containing 2-acyl-lysophosphatidylserine is 40% to 73% by mass.

14. The method according to claim 2 , wherein the percentage of the lysophospholipid in the total phospholipid in the composition containing 2-acyl-lysophosphatidylserine is 40% to 73% by mass.

Assignees

• Yazawa Kazunaga
• Susa Tomoyuki
• Gotoh Shoji
• Tashiro Yasuhito
• Kawashima Junichi
• Imamura Shigeyuki
• Univ Tokyo Nat Univ Corp
• Meiji Co Ltd

Inventors

• Yazawa Kazunaga
• Susa Tomoyuki
• Gotoh Shoji
• Tashiro Yasuhito
• Kawashima Junichi
• Imamura Shigeyuki

Classifications

• A61P25/00

  Drugs for disorders of the nervous system · CPC title

• C12P13/06 Primary

  Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title

• A61K31/685

  one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin · CPC title

• C12P7/6481

  Phosphoglycerides (phosphoglycerides having carboxylic acids with less than seven carbon atoms C12P7/62) · CPC title