Transgenic plants with enhanced agronomic traits

Patent metadata

Field	Value
Publication number	US-9290773-B2
Application number	US-201113199773-A
Country	US
Kind code	B2
Filing date	Sep 8, 2011
Priority date	Oct 2, 2002
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

First claim

What is claimed is:

1. A plant cell nucleus with stably integrated, recombinant DNA, wherein said recombinant DNA comprises a promoter that is functional in said plant cell nucleus and that is operably linked to a protein coding recombinant DNA encoding the protein consisting of SEQ ID NO: 1032 or a protein having an amino acid sequence with at least 95% but less than 100% amino acid sequence identity to SEQ ID NO: 1032; and wherein said plant cell nucleus is selected by screening a population of transgenic plants that have said recombinant DNA and an enhanced trait as compared to control plants that do not have said recombinant DNA in their nuclei; and wherein said enhanced trait is selected from the group of enhanced traits consisting of cold tolerance, yield, nitrogen use efficiency, and seed protein.

2. The plant cell nucleus of claim 1 wherein said protein coding DNA encodes the protein having the amino acid sequence of SEQ ID NO:1032.

3. The plant cell nucleus of claim 1 further comprising a DNA expressing a protein that provides tolerance from exposure to an herbicide applied at levels that are lethal to a wild type plant cell of the same species.

4. The plant cell nucleus of claim 3 wherein said herbicide is a glyphosate, dicamba, or glufosinate compound.

5. A transgenic plant comprising a plurality of plant cells with the plant cell nucleus of claim 1 .

6. The transgenic plant of claim 5 which is homozygous for said recombinant DNA.

7. A transgenic seed comprising a plurality of plant cells with the plant cell nucleus of claim 1 .

8. The transgenic seed of claim 7 , wherein said transgenic seed is obtained from a corn, soybean, cotton, canola, alfalfa, wheat or rice transgenic plant comprising said plant cell nucleus.

9. A transgenic pollen grain comprising a haploid gamete of the plant cell nucleus of claim 1 , wherein the gamete comprises said recombinant DNA.

10. A method for manufacturing non-natural, transgenic seed, said method comprising the steps of:
(a) providing a population of transgenic plants transformed with a recombinant DNA construct comprising a promoter that is functional in a plant cell and that is operably linked to a protein coding recombinant DNA encoding the protein consisting of SEQ ID NO: 1032 or a protein having an amino acid sequence with at least 95% but less than 100% amino acid sequence identity to SEQ ID NO: 1032, wherein said recombinant DNA construct is stably integrated into the genome of said transgenic plants;
(b) screening the population of transgenic plants provided in step (a) for an enhanced trait and said recombinant DNA, wherein said enhanced trait is selected from the group of enhanced traits consisting of water use efficiency, cold tolerance, heat tolerance, resistance to salt exposure, shade tolerance, yield, nitrogen use efficiency, seed protein and seed oil as compared to control plants of the same species lacking said DNA construct;
(c) selecting one or more transformed plants from said screening of step (b) which exhibit said enhanced trait as compared to said control plants lacking said DNA construct, wherein said enhanced trait is due to the expression of said protein in said selected transformed plants;
(d) verifying that said recombinant DNA is stably integrated in said selected transformed plants;
(e) analyzing tissue of said selected transformed plants to determine the expression of a gene that encodes a protein having the function of a protein having the amino acid sequence of SEQ ID NO:1032; and
(f) collecting seed from said selected transformed plants.

11. A method of producing hybrid corn seed comprising:
(a) acquiring hybrid corn seed from a herbicide tolerant corn plant which also has stably-integrated recombinant DNA construct comprising a promoter that is functional in plant cells and that is operably linked to a protein coding recombinant DNA encoding the protein consisting of SEQ ID NO: 1032 or a protein having an amino acid sequence with at least 95% but less than 100% amino acid sequence identity to SEQ ID NO: 1032, wherein said recombinant DNA construct is stably integrated into the genome of said hybrid corn seed;
(b) producing corn plants from said hybrid corn seed of step a), wherein a fraction of the plants produced from said hybrid corn seed is homozygous for said recombinant DNA, a fraction of the plants produced from said hybrid corn seed is hemizygous for said recombinant DNA, and a fraction of the plants produced from said hybrid corn seed has none of said recombinant DNA;
(c) selecting corn plants which are homozygous and hemizygous for said recombinant DNA by treating with an herbicide;
(d) collecting seed from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants;
(e) repeating steps (c) and (d) at least once to produce an inbred corn line; and
(f) crossing said inbred corn line with a second corn line to produce hybrid seed; and wherein said inbred corn line of step (e) and hybrid seed of step (f) comprise said recombinant DNA.

Assignees

• Edgerton Michael D
• Monsanto Technology Llc

Inventors

• Edgerton Michael D

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C07K14/4702 Primary

  Regulators; Modulating activity · CPC title

• A01H5/10

  Seeds · CPC title

• C12N15/8251

  Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis · CPC title

• C12N15/8274

  for herbicide resistance · CPC title