Media and methods for growing mammary organoids
US-2024191187-A1 · Jun 13, 2024 · US
Mammary gland epithelial cell culture
US9290735B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9290735-B2 |
| Application number | US-86658809-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 5, 2009 |
| Priority date | Feb 6, 2008 |
| Publication date | Mar 22, 2016 |
| Grant date | Mar 22, 2016 |
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- Title
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Abstract
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To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.
First claim
Opening claim text (preview).
The invention claimed is: 1. A cell culture method comprising: culturing cells in a multi-layered state in a minute partitioned space and obtaining a tissue structure having a function resembling an in-vivo function, wherein the minute partitioned space is a micro container in a cell culture container having a plurality of micro containers on a surface, wherein adjacent micro containers are communicated with each other with an opening formed in a sidewall partitioning the adjacent micro containers, and a width of the opening is 3 μm to 20 μm, and wherein the cells are mammary gland epithelial cells. 2. The cell culture method according to claim 1 , wherein the cells are primary cells. 3. The cell culture method according to claim 1 , wherein the mammary gland epithelial cells are differentiated and form a hollow acinus structure. 4. The cell culture method according to claim 1 , wherein a base area of the micro container is 9×10 −4 mm 2 to 9×10 −2 mm 2 ; a height of a sidewall is 15 μm to 300 μm; and a width of a sidewall is 3 μm to 15 μm. 5. The cell culture method according to claim 1 , wherein a region of the cell culture container in which a micro container is formed has transparency.
Assignees
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Classifications
Patterned coating · CPC title
Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title
Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title
General methods for three-dimensional culture · CPC title
- C12N5/0631Primary
Mammary cells · CPC title
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- What does patent US9290735B2 cover?
- To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a …
- Who is the assignee on this patent?
- Sokabe Masahiro, Furuya Kishio, Tazaki Go, and 4 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0631. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 22 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).