Peptides that modulate complex SASPase-FLG2

Patent metadata

Field	Value
Publication number	US-9290553-B2
Application number	US-201113992564-A
Country	US
Kind code	B2
Filing date	Dec 5, 2011
Priority date	Dec 7, 2010
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

An isolated peptide from skin aspartic protease (SASPase) or filaggrin-2 (FLG2), a fragment or homologue thereof, which can modify the three-dimensional shape of a complex formed by interaction between a first amino acid sequence from filaggrin-2 and a second amino acid sequence from SASPase, is described. The isolated peptide is useful as a target for screening for cosmetic or therapeutic active agents with regard to the skin and/or its appendages.

First claim

The invention claimed is:

1. A chimeric peptide comprising a first amino acid sequence from filaggrin-2 fused to a second amino acid sequence,
wherein the first amino acid sequence represents a peptide represented by the amino acid sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, and SEQ ID NO: 15, and a homolog having a sequence identity of at least 85% to SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15 and modifies the three-dimensional conformation of a complex formed by interaction of filaggrin-2 with skin aspartic protease.

2. The chimeric peptide as claimed in claim 1 , wherein the second amino acid sequence is selected from the group consisting of a fluorescent protein, an affinity label, a signal peptide and a propenetrating peptide.

3. An isolated complex formed by interaction of a first amino acid sequence from filaggrin-2 with a second amino acid sequence from skin aspartic protease, or a homolog thereof having a sequence identity of at least 85%,
wherein the first amino acid sequence represents a peptide represented by the amino acid sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, and SEQ ID NO: 15, and a homolog having a sequence identity of at least 85% to SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15 and modifies the three-dimensional conformation of a complex formed by interaction of the first amino acid sequence with the second amino acid sequence.

Assignees

• Bernard Dominique
• Thomas-Collignon Agnes
• Oreal

Inventors

• Bernard Dominique
• Thomas-Collignon Agnes

Classifications

• G01N2500/02

  Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor) · CPC title

• G01N33/573

  for enzymes or isoenzymes · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title

• C07K2319/21

  containing a His-tag · CPC title

• C07K14/47 Primary

  from mammals · CPC title