Moveable chromatography column separator

Patent metadata

Field	Value
Publication number	US-9289699-B2
Application number	US-201013386035-A
Country	US
Kind code	B2
Filing date	Jul 28, 2010
Priority date	Jul 30, 2009
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

The current invention is directed to a chromatography column separator which separates the chromatography column in an upper chromatography column chamber and a lower chromatography column chamber, and which has a variable position within the chromatography column, and which is embedded by the chromatography material. The separator allows the replacement of the chromatography material in the upper chromatography column chamber without the need to replace the chromatography column material in the lower chromatography column chamber and it allows also the combination of two different chromatography materials with different chromatographical functional groups in one chromatography column.

First claim

The invention claimed is:

1. A method to purify a polypeptide, comprising
providing a polypeptide to be purified in an aqueous buffered solution,
adding the polypeptide solution to a liquid chromatography column comprising
one or more chromatography column separators comprising
an O-shaped guide ring for use in the chromatography column, wherein the guide ring has a vertical cross-section comprising two axially symmetric cross-section areas, wherein each of the axially symmetric cross-section areas has
i) a tapering structure, wherein the tapering is from the outside to the inside of the guide ring, and
ii) a notch with an opening directed to the inside of the guide ring for mounting a frit, wherein the notch is a rectangular notch,
wherein further each of the cross-section areas has a triangular shape and the longest side has a length of at least 1.5 times the diameter of the notch, and

a frit mounted into the guide ring,

wherein the one or more chromatography column separators divides the chromatography column into chambers,
wherein any chromatography column separator is in contact with a first chromatography material and in contact with a second chromatography material, whereby the first and the second chromatography material are
i) chromatography materials with the same chromatographical functional group and of the same or different particle size, or
ii) chromatography materials with different chromatographical functional groups,

wherein the one or more chromatography column separators are freely moveable within the chromatography column and the chromatography materials,

interaction of the polypeptide with the chromatography materials and obtaining an eluate therefrom, and
recovering the polypeptide from the eluate.

Assignees

• Blaschyk Andreas
• Falkenstein Roberto
• Hoffmann La Roche

Inventors

• Blaschyk Andreas
• Falkenstein Roberto

Classifications

• G01N30/6069

  with compartments or bed substructure · CPC title

• B01D15/22 Primary

  relating to the construction of the column · CPC title

• G01N30/60 Primary

  Construction of the column · CPC title

• G01N30/603

  retaining the stationary phase, e.g. Frits · CPC title