Method and composition for hyperthermally treating cells

Patent metadata

Field	Value
Publication number	US-9289491-B2
Application number	US-201514624334-A
Country	US
Kind code	B2
Filing date	Feb 17, 2015
Priority date	Feb 14, 2002
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

A method and composition for hyperthermally treating tumor cells in a patient under conditions that affect tumor stem cells and tumor cells.

First claim

What is claimed is:

1. A method of providing therapy, the method comprising
administering to a patient in need thereof a plurality of nanoparticles, the nanoparticles coated or otherwise associated with an antibody to specific cells, under conditions sufficient to permit antibody accumulation at a tissue target site,
radiating the target site with an energy source to penetrate into the tissue target site to controllably heat the nanoparticles and generate thermal energy to induce a photoacoustic signal or sound wave from the nanoparticles,
using a processor to control the amount of thermal energy delivered at the desired temperature to the target site,
recording the temperature and photoacoustic signal or sound wave from the target site or from one or more multiple locations, and
amplifying and processing the recorded photoacoustic signal or sound waves to generate a computational tomographic image of the nanoparticles at the tissue target site.

2. The method of claim 1 where imaging generates high-resolution two- or three-dimensional photoacoustic images.

3. The method of claim 1 where the antibody is an anti-tumor antibody, the nanoparticles are attached to tumor cells, and the method generates a two- or three-dimensional photoacoustic image of the tumor.

4. The method of claim 1 where the antibody is directed to a receptor on a normal cell of an organ, the nanoparticles are attached to the normal cells, and the method generates a two- or three-dimensional photoacoustic image of an organ regardless of location of the organ in the body.

5. The method of claim 1 where imaging is by a modality selected from the group consisting of radiofrequency, microwave, ultrasound, focused ultrasound, and combinations thereof.

6. The method of claim 5 where imaging is by photoacoustic temperature imaging combined with a modality selected from the group consisting of ultrasound, focused ultrasound, magnetic resonance imaging (MRI), functional MRI (fMRI), computed tomography (CT), positron emission tomography (PET), OCT, and alternating magnetic field imaging, resulting in enhanced image acquisition and enhanced resolution.

7. The method of claim 1 where the processor communicates the temperature from the photoacoustic source to the energy source to control the amount and duration of energy delivered to a desired temperature.

8. The method of claim 1 where the photoacoustic signal or sound wave is recorded and produced as each of a thermal graph of the target site, and as an image in one-dimension, two-dimensions, or three-dimensions.

9. The method of claim 1 where the energy source to radiate the target site is selected from the group consisting of electromagnetic radiation, ultraviolet radiation, visible light, infrared light, radiofrequency waves, microwaves, focused ultrasound, alternating magnetic field radiation, and combinations thereof.

10. The method of claim 1 where the energy is applied from multiple sites or using multiple energy sources thus minimizing or preventing pain to the patient and overheating of the target site.

11. The method of claim 1 where the energy is applied in a manner selected from the group consisting of continuous, intermittent, oscillatory, pulsed, and combinations thereof.

12. The method of claim 1 where the energy is applied in an oscillatory or pulsed manner thus reducing thermal damage to normal cells while sufficiently heating cells to which the nanoparticles are attached to the desired temperature.

13. The method of claim 1 where the energy is applied intermittently as an alternating magnetic force to heat to the nanoparticle-cell complex, and imaging measures the temperature of the heated tissue and images the target site.

14. The method of claim 1 where the target site is maintained at a temperature selected from the group consisting of 39° C. to 48° C., 37° C. to <60° C., 37° C. to 41° C., 42° C. to 46° C., 47° C. to 50° C., and 50° C. to 58° C.

15. The method of claim 1 where the nanoparticles are coated or otherwise associated with biocompatible molecules selected from the group consisting of PEG, biotin, CPP, ACPP, dendrimers, dendrimers conjugated with poly beta amine, small organic molecules, and combinations thereof.

16. The method of claim 1 provided to the patient in need thereof with a method for enhanced cell penetration of the antibody-nanoparticle complex, the method selected from the group consisting of ultrasound, electroporation, and combinations thereof and resulting in therapy of cells at the target site while sparing normal cells.

17. The method of claim 1 further comprising providing an agent to result in enhanced immunogenicity where the patient in need thereof is undergoing cancer immunotherapy, the agent selected from the group consisting of CHK1, CHK2, CHM1, and CHM2.

18. The method of claim 1 where the patient in need thereof is treated simultaneously or with hemofiltration, hemoadsorption, mesoporous carbide-derived carbon, or another type of agent to prevent a cytokine storm.

19. The method of claim 1 create a photoacoustic image of an internal structure with radiofrequency wave energy or microwave energy alone.

20. The method of claim 10 performed using nanoparticles selected from the group consisting of supramagnetic ferric oxide nanoparticles, supraparamagentic ferric oxide nanoparticles, and combinations thereof.

Assignees

• Peyman Gholam A

Inventors

• Peyman Gholam A

Classifications

• A61F9/0079

  using non-laser electromagnetic radiation, e.g. non-coherent light or microwaves · CPC title

• A61B5/01

  Measuring temperature of body parts {; Diagnostic temperature sensing, e.g. for malignant or inflamed tissue} (clinical contact thermometers G01K13/20) · CPC title

• A61B2019/5234

  Human Necessities · mapped topic

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61B8/14

  Echo-tomography · CPC title