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Promoters for expressing genes in a fungal cell
US9284588B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9284588-B2 |
| Application number | US-201113885145-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 15, 2011 |
| Priority date | Dec 16, 2010 |
| Publication date | Mar 15, 2016 |
| Grant date | Mar 15, 2016 |
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Abstract
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The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.
First claim
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What is claimed is: 1. A method for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a polynucleotide encoding the polypeptide operably linked to a promoter selected from the group consisting of (i) a promoter comprising a nucleotide sequence that has at least 95% sequence identity to SEQ ID NO: 1; and (ii) a promoter comprising SEQ ID NO: 1; wherein the polynucleotide encoding the polypeptide is foreign to the promoter; and (b) isolating the polypeptide from the cultivation medium. 2. The method of claim 1 , wherein the promoter comprises the polynucleotide sequence of SEQ ID NO: 1. 3. The method of claim 1 , wherein one or more portions of the promoter sequence of SEQ ID NO: 1 is contained in a hybrid promoter. 4. The method of claim 1 , wherein the promoter sequence of SEQ ID NO: 1 is contained in a tandem promoter. 5. The method of claim 1 , wherein the polypeptide is foreign to the fungal host cell. 6. The method of claim 1 , wherein the fungal host cell is a filamentous fungal cell. 7. The method of claim 1 , wherein the fungal host cell is a yeast cell. 8. The method of claim 1 , wherein the promoter comprises a nucleotide sequence having at least 96% sequence identity to SEQ ID NO: 1. 9. The method of claim 1 , wherein the promoter comprises a nucleotide sequence having at least 97% sequence identity to SEQ ID NO: 1. 10. The method of claim 1 , wherein the promoter comprises a nucleotide sequence having at least 98% sequence identity to SEQ ID NO: 1. 11. The method of claim 1 , wherein the promoter comprises a nucleotide sequence having at least 99% sequence identity to SEQ ID NO: 1.
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Frequently asked questions
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- What does patent US9284588B2 cover?
- The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.
- Who is the assignee on this patent?
- Merino Sandra, Yaver Debbie, Novozymes Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N15/80. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 15 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).