Strain classified under Botryococcus braunii

US9284577B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9284577-B2
Application numberUS-201113992590-A
CountryUS
Kind codeB2
Filing dateDec 9, 2011
Priority dateDec 9, 2010
Publication dateMar 15, 2016
Grant dateMar 15, 2016

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  1. Title

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  2. Abstract

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  4. Key dates

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  5. First independent claim

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Abstract

Official abstract text for this publication.

Provision of a novel strain belonging to Botryococcus braunii , capable of growth under a wide range of culturing conditions, having a high produced hydrocarbon content, and having high purity of the target hydrocarbon.

First claim

Opening claim text (preview).

What is claimed is: 1. A method for producing a hydrocarbon from a strain belonging to Botryococcus braunii race-B that produces a hydrocarbon of the molecular formula C 34 H 58 at 60% by weight or greater with respect to the total produced hydrocarbons, comprising the steps of culturing said strain at a temperature of 35° C. to 50° C., and then extracting said hydrocarbon from said cultured strain with at least one organic solvent, wherein said strain is Botryococcus braunii tsukuba-1 (deposit number: FERM ABP-11441). 2. The method according to claim 1 , wherein the culturing is conducted at a temperature of 39° C. 3. The method according to claim 1 , wherein the culturing is conducted at a temperature of 36° C. 4. The method according to claim 1 , wherein the hydrocarbon of the molecular formula C 34 H 58 has the structure of the following Formula (I): 5. The method of claim 1 , wherein the culturing is conducted at a temperature of 35° C. to 40° C. 6. The method of claim 5 , wherein the culturing is conducted at a temperature of 35° C. to 36° C. 7. The method of claim 1 , wherein the at least one organic solvent is n-hexane. 8. The method of claim 1 , wherein the at least one organic solvent is a mixture of chloroform and methanol. 9. The method of claim 8 , wherein the at least one organic solvent is a 1:1 mixture of chloroform and methanol. 10. The method of claim 8 , wherein the at least one organic solvent is a 1:2 mixture of chloroform and methanol. 11. The method of claim 8 , wherein the hydrocarbon extracted is concentrated to dryness under a nitrogen stream and again extracted with n-hexane. 12. The method of claim 1 , wherein the culturing is conducted for a period of 1 to 5 months. 13. The method of claim 12 , wherein the culturing is conducted for a period of 1 to 3 months.

Assignees

Inventors

Classifications

  • C12N1/12Primary

    Unicellular algae; Culture media therefor (as new plants A01H13/00) · CPC title

  • C12P5/026Primary

    Unsaturated compounds, i.e. alkenes, alkynes or allenes · CPC title

  • containing one or more isoprene units, i.e. terpenes (carotenes C12P23/00) · CPC title

  • Chemistry & Metallurgy · mapped topic

  • Algae {; Processes using algae} · CPC title

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What does patent US9284577B2 cover?
Provision of a novel strain belonging to Botryococcus braunii , capable of growth under a wide range of culturing conditions, having a high produced hydrocarbon content, and having high purity of the target hydrocarbon.
Who is the assignee on this patent?
Watanabe Makoto, Kaya Kunimitsu, Tanoi Takako, and 3 more
What technology area does this patent fall under?
Primary CPC classification C12N1/12. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Mar 15 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).