Immunoglobulin-binding polypeptide

Patent metadata

Field	Value
Publication number	US-9284354-B2
Application number	US-201214007211-A
Country	US
Kind code	B2
Filing date	Mar 26, 2012
Priority date	Mar 25, 2011
Publication date	Mar 15, 2016
Grant date	Mar 15, 2016

Abstract

An object of the present invention is to provide novel polypeptides that are capable of binding to an immunoglobulin and have high stability against alkali. The present invention relates to proteins having the amino acid sequence of SEQ ID No:1 or 2.

First claim

The invention claimed is:

1. A polypeptide, that binds to a protein comprising an Fc region of an immunoglobulin, the polypeptide comprising the following amino acid sequence:

(SEQ ID NO: 47)

RFX 1 X 2 EQQNAFYEILHX 3 PNLTEEQRNX 4 FIQX 5 LX 6 X 7 X 8 PSVSREX 9 L

AEAX 10 X 11 LNDAQAPX 12

wherein X 1 is D, E, N, or Q;
X 2 is E or R;
X 3 is L, M, or I;
X 4 is A, E, F, R, Y, or W;
X 5 is D, E, H, I, L, Q, R, S, T, or V;
X 6 is H, I, or R;
X 7 is D, I, or R;
X 8 is D or E;
X 9 is I, L, or V;
X 10 is R or Q;
X 11 is H or R; and
X 12 is R, G, or K.

2. A polypeptide, that binds to a protein comprising an Fc region of an immunoglobulin, the polypeptide comprising the following amino acid sequence:

(SEQ ID NO: 48)

Z 1 Z 2 Z 3 RFX 1 X 2 EQQNAFYEILHX 3 PNLTEEQRNX 4 FIQX 5 LX 6 X 7 X 8 PSVS

REX 9 LAEAX 10 X 11 LNDAQAPX 12

wherein X 1 is D, E, N, or Q;
X 2 is E or R;
X 3 is L, M, or I;
X 4 is A, E, F, R, Y, or W;
X 5 is D, E, H, I, L, Q, R, S, T, or V;
X 6 is H, I, or R;
X 7 is D, I, or R;
X 8 is D or E;
X 9 is I, L, or V;
X 10 is R or Q;
X 11 is H or R;
X 12 is R, G, or K; and
Z 1 to Z 3 are each independently A, D, E, G, H, I, L, N, Q, R, S, T, V, or Y.

3. The polypeptide according to claim 1 ,
which has at least 90% sequence identity to one of the following amino acid sequences:

(SEQ ID No: 1)

ADNRFNREQQNAFYEILHLPNLTEEQRNAFIQSLRDDPSVSREILAEAR

RLNDAQAPG

and

(SEQ ID No: 2)

ADNRFNREQQNAFYEILHLPNLTEEQRNAFIQSLRDDPSVSREILAEAR

RLNDAQAPR.

4. The polypeptide according to claim 2 ,
which has at least 90% sequence identity to one of the following amino acid sequences:

(SEQ ID No: 1)

ADNRFNREQQNAFYEILHLPNLTEEQRNAFIQSLRDDPSVSREILAEAR

RLNDAQAPG

and

(SEQ ID No: 2)

ADNRFNREQQNAFYEILHLPNLTEEQRNAFIQSLRDDPSVSREILAEAR

RLNDAQAPR.

Assignees

• Yoshida Shinichi
• Murata Dai
• Taira Shunichi
• Kaneka Corp

Inventors

• Yoshida Shinichi
• Murata Dai
• Taira Shunichi

Classifications

• C07K14/47

  from mammals · CPC title

• C07K14/00 Primary

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C07K14/31 Primary

  from Staphylococcus (G) · CPC title

• C12N15/09 Primary

  Recombinant DNA-technology · CPC title