Sorting particles using high gradient magnetic fields

Patent metadata

Field	Value
Publication number	US-9278353-B2
Application number	US-201314410985-A
Country	US
Kind code	B2
Filing date	Jun 25, 2013
Priority date	Jun 25, 2012
Publication date	Mar 8, 2016
Grant date	Mar 8, 2016

Abstract

This disclosure describes microfluidic devices that include one or more magnets, each magnet being operable to emit a magnetic field; and a magnetizable layer adjacent to the one or more magnets, in which the magnetizable layer is configured to induce a gradient in the magnetic field of at least one of the magnets. For example, the gradient can be at least 10 3 T/m at a position that is at least 20 μm away from a surface of the magnetizable layer. The magnetizable layer includes a first high magnetic permeability material and a low magnetic permeability material arranged adjacent to the high magnetic permeability material. The devices also include a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the high magnetic permeability material and the low magnetic permeability material.

First claim

What is claimed is:

1. A microfluidic device comprising:
one or more magnets, wherein each magnet is operable to emit a magnetic field;
a magnetizable layer arranged adjacent to the one or more magnets, wherein the magnetizable layer is configured to induce a gradient in the magnetic field of at least one of the magnets, wherein the gradient is at least 10 3 T/m at a position that is at least 20 μm away from a surface of the magnetizable layer, and wherein the magnetizable layer comprises
a first high magnetic permeability material, and
a low magnetic permeability material arranged adjacent to or at least partially bordering the high magnetic permeability material; and

a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the first high magnetic permeability material and the low magnetic permeability material.

2. The microfluidic device of claim 1 , further comprising a second high magnetic permeability material arranged at a distance from the first high magnetic permeability material to form a gap between them, wherein the gap is filled with low magnetic permeability material.

3. The microfluidic device of claim 1 , wherein the gradient is at least 10 4 T/m at a position of at least 20 μm away from the surface of the magnetizable layer.

4. The microfluidic device of claim 1 , further comprising a low magnetic permeability spacer layer between the one or more magnets and the magnetizable layer.

5. The microfluidic device of claim 2 , wherein a thickness of the gap is equal to or less than a thickness of each of the first and second high magnetic permeability material, and a width of the gap is equal to or greater than approximately 100 nm.

6. The microfluidic device of claim 1 , wherein the angle is an oblique angle.

7. The microfluidic device of claim 1 , wherein the first high magnetic permeability material is selected from the group consisting of iron, nickel, cobalt, Nickel-Iron alloy, SiFe alloy, FeAlN alloy, a CoFe alloy, CoFeNi, steel, a polymer composite containing magnetic particles, a glass composite containing magnetic particles, and a ceramic composite containing magnetic particles.

8. The microfluidic device of claim 1 , wherein the low magnetic permeability material comprises a polymer or air.

9. The microfluidic device of claim 1 , comprising a passivation layer arranged on the surface of the magnetizable layer and forming a bottom surface to the microfluidic channel.

10. The microfluidic device of claim 1 , comprising an array of two or more magnets, each magnet having a magnetic pole orientation that is opposite to a magnetic pole orientation of an adjacent magnet in the array such that the magnetic field of each magnet in the array extends to an adjacent magnet in the array.

11. The microfluidic device of claim 10 , wherein the low magnetic permeability material is substantially aligned with an interface between two adjacent magnets of the array.

12. The microfluidic device of claim 1 , further comprising:
a deflection channel; and
an output channel separate from the deflection channel, wherein both the output channel and the deflection channel are fluidly coupled to an outlet of the microfluidic channel.

13. The microfluidic device of claim 1 , wherein the magnetizable layer comprises a plurality of pieces of the low magnetic permeability material, wherein each piece of the low magnetic permeability material is arranged within a corresponding elongated gap in the first high magnetic permeability material or between separate pieces of the first high magnetic permeability material.

14. A microfluidic device comprising:
one or more magnets, wherein each magnet is operable to emit a magnetic field;
a magnetizable layer arranged on a surface of the one or more magnets, wherein the magnetizable layer comprises a high magnetic permeability material and a low magnetic permeability material adjacent to or at least partially bordering the high magnetic permeability material, wherein a thickness of the high magnetic permeability material is greater than 10 μm and a saturation flux density of the high magnetic permeability material is greater than 0.2 T, wherein the magnetizable layer is configured to induce a gradient in the magnetic field of at least one of the magnets; and
a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from a an interface between the low magnetic permeability material and the high magnetic permeability material.

15. The microfluidic device of claim 14 , further comprising:
a deflection channel; and
an output channel separate from the deflection channel, wherein both the output channel and the deflection channel are fluidly coupled to an outlet of the microfluidic channel.

16. A microfluidic cartridge comprising:
a magnetizable layer, the magnetizable layer comprising a high magnetic permeability material and a low magnetic permeability material adjacent to or at least partially bordering the high magnetic permeability material;
a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the high magnetic permeability material and the low magnetic permeability material; and
a passivation layer arranged on the surface of the magnetizable layer and forming a bottom surface to the microfluidic channel,
wherein a surface of the magnetizable layer is configured to be removably secured to a one or more magnets.

17. The microfluidic cartridge of claim 16 , wherein the surface of the magnetizable layer comprises protruding structures configured to be removably locked into corresponding sections of the one or more magnets.

18. The microfluidic cartridge of claim 16 , wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the low magnetic permeability material and the high magnetic permeability material.

19. The microfluidic cartridge of claim 16 , wherein the low magnetic permeability material is arranged within a gap in the high magnetic permeability material or between separate pieces of the high magnetic permeability material.

20. The microfluidic cartridge of claim 16 , wherein a first surface of the high magnetic permeability material and a first surface of the low magnetic permeability material are in direct contact with the passivation layer, and wherein a thickness of the low magnetic permeability material as measured from the passivation layer is greater than a thickness of the high magnetic permeability material.

Assignees

• Gen Hospital Corp

Inventors

• Smith Kyle C
• Haghgooie Ramin
• Barber Thomas Alan
• Ozkumur Ismail Emre
• Kapur Ravi
• Toner Mehmet

Classifications

• B03C1/288 Primary

  disposed at the outer circumference of a recipient · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title

• G01N1/4077

  by other techniques involving separation of suspended solids · CPC title

• B01L2400/043

  magnetic forces · CPC title