Pipetting device, modular pipetting unit, pipetting system and method for pipetting of fluid samples

Patent metadata

Field	Value
Publication number	US-9278349-B2
Application number	US-201414528182-A
Country	US
Kind code	B2
Filing date	Oct 30, 2014
Priority date	Nov 28, 2008
Publication date	Mar 8, 2016
Grant date	Mar 8, 2016

Abstract

A pipetting device having a modular pipetting unit including a pipetting tip for pipetting of fluid samples and a pump conduit for transferring a negative or positive pressure to the pipetting tip is disclosed. The pipetting tip and a portion of the pump conduit adjoining the pipetting tip mutually define a fluid sample conduit for receiving the fluid samples. The modular pipetting unit is detachably attached to an automated positioning device for positioning the modular pipetting unit. A system and method for pipetting of fluid samples using such a pipetting device are also disclosed wherein pipetting of the fluid samples is performed in such a manner that each pipetted fluid sample volume is smaller than a volume of the fluid sample conduit.

First claim

What is claimed is:

1. A pipetting device for an automated positioning device, comprising:
at least one modular pipetting unit which includes a pipetting tip for pipetting of fluid samples;
a pump conduit tot transferring a negative or positive pressure to said pipetting-tip,
a hollow housing comprising an inner wall surrounding a cavity and a plurality of guiding faces arranged along the inner wall guiding the pump conduit, said pump conduit comprising a first vertical conduit portion extending in the direction of gravity, a second horizontal conduit portion, and a first curved conduit portion connecting said first vertical conduit portion to said second horizontal conduit portion, said first vertical conduit portion being longer than the second horizontal conduit portion;
wherein said pipetting tip and at least a portion of said pump conduit adjoining said pipetting tip mutually define a fluid sample conduit for receiving said fluid samples, and
wherein said modular pipetting unit is detachably attachable to the automated positioning device which positions said modular pipetting unit.

2. The pipetting device according to claim 1 , wherein said modular pipetting unit has an integrated pump connected to said pipetting tip by means of said pump conduit.

3. The pipetting device according to claim 2 , wherein said pump is a pump of the rotary displacement pump type such as a gearwheel pump.

4. The pipetting device according to claim 1 , wherein said fluid sample conduit and pipetting tip have an at least approximate vertical orientation with respect to gravity.

5. The pipetting device according to claim 1 , wherein said modular pipetting unit comprises at least one sensor for sensing of physical parameters selected from the group consisting of flow rate, liquid pressure, and distance.

6. The pipetting device according to claim 1 , wherein said housing is provided with at least one connector selected from the group consisting of a fluidic connector and an electric connector.

7. The pipetting device according to claim 1 , comprising a plurality of modular pipetting units having a plurality of equally-distanced pipetting tips.

8. A modular pipetting unit comprising:
at least one pipetting tip for pipetting of fluid samples;
a pump conduit tot transferring a negative or positive pressure to said at least one pipetting tip; and
a hollow housing comprising an inner wall surrounding a cavity and a plurality of guiding faces arranged along the inner wall guiding the pump conduit, said pump conduit comprising a first vertical conduit portion extending in the direction of gravity, a second horizontal conduit portion, and a first curved conduit portion connecting said first vertical conduit portion to said second horizontal conduit portion, said first vertical conduit portion being longer than the second horizontal conduit portion;
wherein said at least one pipetting tip and at least a portion of said pump conduit adjoining said at least one pipetting tip mutually define a fluid sample conduit for accommodating said fluid samples.

9. A pipetting system, comprising:
at least one pipetting unit which includes a pipetting tip for pipetting fluid samples and a pump conduit for transferring a negative or positive pressure to said pipetting tip, the at least one pipetting unit comprising a hollow housing comprising an inner wall surrounding a cavity and a plurality of guiding faces arranged along the inner wall guiding the pump conduit, said pump conduit comprising a first vertical conduit portion extending in the direction of gravity, a second horizontal conduit portion, and a first curved conduit portion connecting said first vertical conduit portion to said second horizontal conduit portion, said first vertical conduit portion being longer than the second horizontal conduit portion, wherein said pipetting tip and at least a portion of said pump conduit adjoining said pipetting tip mutually define a fluid sample conduit for receiving said fluid samples;
an automated positioning device for positioning of the at least one pipetting unit; and
a controller for controlling pipetting of said fluid samples such that each pipetted volume of the fluid samples is smaller than a volume of said fluid sample conduit.

10. The pipetting system according to claim 9 , wherein said at least one pipetting unit is a modular pipetting unit being detachably attached to said automated positioning device.

11. The pipetting system according to claim 10 , wherein said modular pipetting unit comprises a pump connected to said pipetting tip by said pump conduit.

12. The pipetting system according to claim 9 , comprises a liquid system fluid reservoir, wherein said pump conduit is fluidically connected to the liquid system fluid reservoir containing a liquid system fluid and the pump conduit is filled with an amount of the liquid system fluid.

13. The pipetting system according to claim 9 , wherein said fluid sample conduit and pipetting tip have an at least approximate vertical orientation with respect to gravity.

14. The pipetting system according to claim 9 , wherein said pipetting unit comprises at least one sensor for sensing of physical parameters selected from the group consisting of flow rate, liquid pressure, and distance.

15. The pipetting system according to claim 9 , wherein said housing is provided with at least one connector selected from the group consisting of a fluidic connector and an electric connector.

Assignees

• Roche Molecular Systems Inc

Inventors

• Haack Carsten
• Engel Thomas
• Holenstein Tobias

Classifications

• G01N35/1011

  Control of the position or alignment of the transfer device · CPC title

• B01L2400/0475

  specific mechanical means and fluid pressure · CPC title

• B01L3/021 Primary

  Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids · CPC title

• Y10T436/2575

  Volumetric liquid transfer · CPC title

• G01N35/1072

  with provision for selective pipetting of individual channels · CPC title