Purification of succinic acid from fermentation broth on non-functionalized chromatographic resin

Patent metadata

Field	Value
Publication number	US-9272977-B2
Application number	US-201314399028-A
Country	US
Kind code	B2
Filing date	Apr 17, 2013
Priority date	May 7, 2012
Publication date	Mar 1, 2016
Grant date	Mar 1, 2016

Abstract

A process for purifying a filtered fermentation broth of succinic acid is described. The process involves separating succinic acid from the other organic acids and salts in a raw filtered broth using a non-functionalized chromatographic resin. The operation can be run either as an isocratic elution to yield free succinic acid, or as a gradient or binary elution to produce succinate salt.

First claim

We claim:

1. A process for obtaining a succinate product from a fermentation broth comprising:
filtering the fermentation broth to obtain a succinate-containing filtrate;
ensuring that the succinate-containing filtrate has a pH of about 3.0 or less;
running said succinate-containing filtrate through a chromatographic column having a non-functionalized resin at an operational temperature in a range of about 20° C. to about 100° C.,
wherein said operational temperature is selected to produce a column eluent that is selectively either enriched in free succinic acid relative to a salt of succinic acid, or enriched in a salt of succinic acid relative to free succinic acid.

2. The process according to claim 1 , wherein said operational temperature is either a) greater than 50° C. to produce said eluent enriched in free succinic acid, or b) less than 50° C. to produce said eluent enriched in said salt of succinic acid.

3. The process according to claim 2 , wherein said operational temperature for producing said eluent enriched in free succinic acid is between about 50° C. to about 90° C.

4. The process according to claim 2 , wherein said operational temperature for producing said eluent enriched in said salt of succinic acid is between about 20° C., to about 45° C.

5. The process according to claim 1 , further comprising crystallizing succinic acid from said eluent enriched in free succinic acid.

6. The process according to claim 5 , wherein said crystallized succinic acid exhibits >90% purity after a single crystallization.

7. The process according to claim 1 , wherein said eluent enriched in free succinic acid manifests as a distinct chromatographic peak within 3 bed volumes of elution of said column.

8. The process according to claim 1 , wherein said succinate-containing filtrate has a pH of about 1.0 to about 3.0.

9. The process according to claim 1 , wherein producing said eluent enriched in free succinic acid involves an isocratic elution of said column with deionized water.

10. The process according to claim 1 , wherein producing said eluent enriched in said salt of succinic acid involves a binary elution of said column with either a) deionized water and aqueous strong base, or b) deionized water and an organic solvent.

11. The process according to claim 1 , wherein said non-functionalized resin is a hydrophobic polystyrene-di-vinylbenzene (PS-DVB).

12. The process according to claim 1 , wherein said chromatographic column is configured in a simulated-moving bed (SMB) chromatographic system.

13. A method for producing a succinate salt comprising: filtering a fermentation broth to yield a succinate-containing filtrate; ensuring that said filtrate has a pH of 3.0 or less; processing said filtrate through a liquid chromatographic column over a non-functionalized resin at a temperature of up to 70° C., and eluting said chromatographic column with a strong base or organic solvent to obtain an eluent enriched with a succinic salt.

14. The process according to claim 13 , wherein said temperature is between about 20° C. and about 45° C.

15. A method of purifying succinic acid from a fermentation broth comprising: filtering a fermentation broth to yield a clarified broth filtrate; ensuring said clarified broth filtrate has a pH of about 3.0 or less; introducing said clarified broth filtrate into a continuous simulated moving bed chromatographic apparatus configured with column comprising a non-functionalized resin, at an operational temperature selected to produce a column eluent that is selectively enriched in free succinic acid relative to a salt of succinic acid; and crystallizing said free succinic acid from said column eluent.

16. The method according to claim 15 , wherein said crystalized free succinic acid has at least 90% purity after a single crystallization.

17. The method according to claim 15 , wherein said column eluent of free succinic acid manifests as a distinct chromatographic peak within 3 bed volumes of elution of said column.

18. The method according to claim 17 , wherein said column eluent of free succinic acid manifests as a distinct peak between about 1.0 and 2.75 bed volumes of elution of said column.

19. The method according to claim 15 , wherein said column is eluted by an isocratic elution.

20. The method according to claim 15 , wherein said operational temperature is between about 50° C. and 100° C.

Assignees

• Archer Daniels Midland Co

Inventors

• Soper John G
• Schultz Mitchell
• Binder Thomas P

Classifications

• B01D15/426

  Specific type of solvent · CPC title

• C07C51/47 Primary

  by solid-liquid treatment; by chemisorption · CPC title

• B01D15/327

  with hydrophobic interaction · CPC title

• C12P7/46

  Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid · CPC title

• C07C51/42 Primary

  Separation; Purification; Stabilisation; Use of additives · CPC title