Method for purifying carboxylic acids from fermentation broths

Patent metadata

Field	Value
Publication number	US-9272976-B2
Application number	US-201214363844-A
Country	US
Kind code	B2
Filing date	Nov 15, 2012
Priority date	Dec 9, 2011
Publication date	Mar 1, 2016
Grant date	Mar 1, 2016

Abstract

Disclosed is a method for removing and purifying carboxylic acids from fermentation broths, comprising removing biomass and any solids present from the fermentation broth, finely cleaning up the biomass-free and solids-free fermentation broth by nanofiltration, and removing the carboxylic acid from the finely cleaned, biomass-free, and solids free fermentation broth by adsorption to one or more solid phases having tertiary amino groups.

First claim

The invention claimed is:

1. A method for removing and purifying carboxylic acids from fermentation broths by use of an adsorption material disposed in an adsorption column, comprising:
removing biomass and any solids present from the fermentation broth;
nanofiltering the biomass-free and solids-free fermentation broth to remove impurities therefrom and reduce an amount of the impurities that may contact and be adsorbed into the adsorption material in the adsorption column, thereby increasing a rate of production of carboxylic acid; and
removing the carboxylic acid from the nanofiltered, biomass-free, and solids free fermentation broth by adsorption to one or more solid phases of adsorption material having tertiary amino groups.

2. The method of claim 1 , wherein said removing biomass from the fermentation broth is achieved by at least one of precoat filtration, microfiltration, or ultrafiltration.

3. The method of claim 2 , further comprising:
recycling the removed biomass back to the fermenter.

4. The method of claim 2 , wherein said removing biomass from the fermentation broth is achieved without lowering the pH of the fermentation broth and without thermal inactivation of the biomass.

5. The method of claim 2 , wherein during said step of removing biomass and any solids present, an elapsed time between an end of fermentation and the removal of the biomass is no more than 2 hours.

6. The method of claim 2 , wherein a biomass concentration in a filtrate from the at least one of precoat filtration, microfiltration, or ultrafiltration is not greater than 1 g/l.

7. The method of claim 1 , wherein said nanofiltering step utilizes nanofiltration membranes having a separation cut-off of from about 100 to about 400 Da.

8. The method of claim 1 , further comprising:
subjecting the nanofiltered fermentation broth to reverse osmosis.

9. The method of claim 1 , wherein the tertiary amino groups are pyridine groups selected from the group consisting of polyvinylpyridine, poly-2-vinylpyridines, and poly-4-vinylpyridines.

10. The method of claim 1 , wherein the one or more solid phases of adsorption material used for adsorption is a polymer crosslinked with divinylbenzene.

11. The method of claim 1 , wherein the one or more solid phases of adsorption material used for adsorption is formed from one or more different polymer materials.

12. The method of claim 1 , wherein the carboxylic acids to be removed and purified are selected from the group consisting of hydroxycarboxylic acids and dicarboxylic acids.

13. The method of claim 12 , wherein the hydroxycarboxylic acid is selected from the group consisting of malic acid, glycolic acid, isocitric acid, mandelic acid, lactic acid, tartronic acid, tartaric acid, citric acid, β-hydroxybutyric acid, mevalonic acid and salicylic acid.

14. The method of claim 12 , wherein the dicarboxylic acid is selected from the group consisting of oxalic acid, maleic acid, succinic acid, glutaric acid, adipic acid, pimelic acid, suberic acid, fumaric acid and itaconic acid.

15. A device for removing and purifying carboxylic acids from fermentation broths, comprising:
means for removing biomass and any solids present from the fermentation broth;
a nanofiltration device in fluid communication with the means for removing biomass from the fermentation broth, said nanofiltration device being configured to reduce an amount of the impurities that may contact and be adsorbed into the adsorption material in the adsorption column, thereby increasing a rate of production of carboxylic acid; and
means for removing the carboxylic acid from the finely cleaned, biomass-free, and solids free fermentation broth by adsorption to one or more solid phases having tertiary amino groups.

16. The method of claim 1 , wherein said nanofiltering step utilizes nanofiltration membranes having a separation cut-off of 200 Da.

17. The method of claim 12 , wherein the hydroxycarboxylic acid is lactic acid.

Assignees

• Schulze Joachim
• Tietz Wolfgang
• Waengler Isabel
• Kuehlein Klaus
• Thyssenkrupp Ind Solutions Ag

Inventors

• Schulze Joachim
• Tietz Wolfgang
• Waengler Isabel
• Kuehlein Klaus

Classifications

• C07C57/15

  Fumaric acid · CPC title

• C07C59/255

  Tartaric acid · CPC title

• C07C59/153

  Glyoxylic acid · CPC title

• C07C51/42

  Separation; Purification; Stabilisation; Use of additives · CPC title

• C07C59/08

  Lactic acid · CPC title