Polypeptides having endoglucanase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9267126-B2
Application number	US-201113817783-A
Country	US
Kind code	B2
Filing date	Aug 30, 2011
Priority date	Aug 30, 2010
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

What is claimed is:

1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having endoglucanase activity comprises:
an amino acid sequence having at least 95% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

2. A recombinant host cell comprising the nucleic acid construct of claim 1 .

3. A method of producing a polypeptide having endoglucanase activity, said method comprising:
(a) cultivating the host cell of claim 2 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

4. A transgenic plant, plant part or plant cell transformed with the nucleic acid construct of claim 1 .

5. A method of producing a polypeptide having endoglucanase activity, said method comprising:
(a) cultivating the transgenic plant or plant cell of claim 4 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

6. A process for degrading or converting a cellulosic material, said process comprising:
(a) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity; and
(b) recovering the degraded or converted material;
wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 95% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

7. A process for producing a fermentation product, said process comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 95% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

8. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 97% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

9. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 98% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

10. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 99% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

11. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises the amino acid sequence of SEQ ID NO: 2.

12. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having amino acids 18 to 343 of SEQ ID NO: 2.

13. A recombinant expression vector comprising the nucleic acid construct of claim 1 .

14. The process of claim 6 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 97% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

15. The process of claim 6 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having amino acids 18 to 343 of SEQ ID NO: 2.

16. The process of claim 7 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 97% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

17. The process of claim 7 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having amino acids 18 to 343 of SEQ ID NO: 2.

18. An isolated recombinant host cell transformed with a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is heterologous to the recombinant host cell, and wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 95% sequence identity to amino acids 18 to 343 of SEQ ID NO: 2.

19. The recombinant host cell of claim 18 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having at least 97% sequence identity to mature polypeptide of SEQ ID NO: 2.

20. The recombinant host cell of claim 18 , wherein the polypeptide having endoglucanase activity comprises the amino acid sequence of SEQ ID NO: 2.

21. The recombinant host cell of claim 18 , wherein the polypeptide having endoglucanase activity comprises an amino acid sequence having amino acids 18 to 343 of SEQ ID NO: 2.

Assignees

• Spodsberg Nikolaj
• Novozymes Inc

Inventors

• Spodsberg Nikolaj

Classifications

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12N9/2437 Primary

  Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title