Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9267125-B2
Application number	US-201013505939-A
Country	US
Kind code	B2
Filing date	Nov 5, 2010
Priority date	Nov 6, 2009
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

What is claimed is:

1. A method for degrading or converting a cellulosic material, comprising: treating the cellulosic material with a polypeptide having cellobiohydrolase activity, wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to amino acids 17 to 447 of SEQ ID NO: 2.

2. The method of claim 1 , further comprising recovering the degraded cellulosic material.

3. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 95% sequence identity to amino acids 17 to 446 of SEQ ID NO: 2.

4. A recombinant expression vector comprising the nucleic acid construct of claim 3 .

5. A recombinant host cell comprising the nucleic acid construct of claim 3 .

6. A transgenic plant, plant part or plant cell transformed with the nucleic acid construct of claim 3 .

7. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 96% sequence identity to amino acids 17 to 447 of SEQ ID NO: 2.

8. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 97% sequence identity to amino acids 17 to 447 of SEQ ID NO: 2.

9. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 98% sequence identity to amino acids 17 to 447 of SEQ ID NO: 2.

10. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 99% sequence identity to amino acids 17 to 447 of SEQ ID NO: 2.

11. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises amino acids 17 to 447 of SEQ ID NO: 2.

12. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises the amino acid sequence of SEQ ID NO: 2.

13. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide comprising a nucleic acid sequence having at least 96% sequence identity to nucleotides 109 to 1401 of SEQ ID NO: 1.

14. The nucleic acid construct of claim 13 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide comprising a nucleic acid sequence having at least 97% sequence identity to nucleotides 109 to 1401 of SEQ ID NO: 1.

15. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide comprising a nucleic acid sequence having at least 98% sequence identity to nucleotides 109 to 1401 of SEQ ID NO: 1.

16. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide comprising a nucleic acid sequence having at least 99% sequence identity to nucleotides 109 to 1401 of SEQ ID NO: 1.

17. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide comprising nucleotides 109 to 1401 of SEQ ID NO: 1.

18. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1.

19. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide contained in plasmid pMStr199 which is contained in E. coli DSM 23379.

20. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity is a variant comprising a substitution, deletion and/or insertion of one or more amino acids of amino acids 17 to 447 of SEQ ID NO: 2.

21. A method of producing a polypeptide having cellobiohydrolase activity, comprising: (a) cultivating the recombinant host cell of claim 5 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

22. A method of producing a polypeptide having cellobiohydrolase activity, comprising: (a) cultivating the transgenic plant or the plant cell of claim 6 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

23. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to amino acids 17 to 447 of SEQ ID NO: 2.

24. The method of claim 1 , wherein the polypeptide having cellobiohydrolase comprises amino acids 17 to 447 of SEQ ID NO: 2.

25. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity is a variant comprising a substitution, deletion and/or insertion of one or more amino acids of amino acids 17 to 447 of SEQ ID NO: 2.

Assignees

• Vlasenko Elena
• Mcbrayer Brett
• Stringer Mary
• Schnorr Kirk
• Novozymes Inc

Inventors

• Vlasenko Elena
• Mcbrayer Brett
• Stringer Mary
• Schnorr Kirk

Classifications

• C07K14/37

  from fungi · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P19/12

  Disaccharides · CPC title

• C12Y302/01091

  Cellulose 1,4-beta-cellobiosidase (3.2.1.91) · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title