Nanotube structures, methods of making nanotube structures, and methods of accessing intracellular space

Patent metadata

Field	Value
Publication number	US-9266725-B2
Application number	US-201213455245-A
Country	US
Kind code	B2
Filing date	Apr 25, 2012
Priority date	Apr 27, 2011
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.

First claim

We claim the following:

1. A nanotube device, comprising:
a porous structure having a pore diameter of between 100-750 nm and a plurality of nanotubes extending through the porous structure, wherein the nanotubes extend a distance above the porous structure and are hollow to allow material to pass through the nanotubes and have an outer diameter of between 100-750 nm wherein the nanotubes of the porous structure are in fluidic communication with a fluidic passage of a device on a side opposite the nanotubes extending from a surface of the porous structure;
further wherein a density of the nanotubes is between about 10 6 and 10 8 nanotubes/cm 2 .

2. The nanotube device of claim 1 , further comprising a layer of material disposed on a bottom of the porous structure on the side opposite the nanotubes extending from the surface of the porous structure, wherein a material of the layer of material and the nanotubes are made of the same material.

3. The nanotube device of claim 1 , further comprising a holding structure, wherein the porous structure is a bottom surface of the holding structure, wherein walls form side boundaries of the holding structure, and wherein the nanotubes extending above the porous structure surface extend up into an area bounded by the walls and the porous structure.

4. The nanotube device of claim 1 , wherein the porous structure is made of a material that is different than the nanotubes.

5. The nanotube device of claim 1 , wherein the distance is about 100 nm to 10 μm.

6. The nanotube device of claim 1 , wherein the porous structure is made of a material selected from the group consisting of: polycarbonate, polyester, a polymer, an etchable material that can be processed with pores, silicon, and a combination thereof.

7. The nanotube device of claim 1 , wherein the nanotubes are made of a material selected from the group consisting of: alumina, TiO 2 , SnO 2 , ZrO 2 , ZnO 2 , carbon, a nitride, platinum, gold, silver, indium tin oxide (ITO), SiO 2 , Ni, NiO, and a combination thereof.

8. The nanotube device of claim 1 , wherein the porous structure has a thickness of about 100 nm to 50 μm.

9. The nanotube device of claim 1 , further wherein the fluidic passage is configured for rapid fluid exchange as compared to flow through the nanotubes.

10. A nanotube device, the device comprising:
a porous structure having a pore diameter of between 100-750 nm and a plurality of nanotubes extending through the porous structure and a distance above the porous structure at a density of between about 10 6 and 10 8 nanotubes/cm 2 , wherein the nanotubes have an outer diameter of between 100-750 nm;
a fluidic passage on a side of the porous structure opposite from a side the nanotubes extend above the porous structure, wherein the nanotubes are in fluidic communication with the fluidic passage so that a material can pass from the fluidic passage through the nanotubes.

11. The device of claim 10 , further comprising a layer of material disposed on a bottom of the porous structure on the side opposite the nanotubes extending from a surface of the porous structure, wherein a material of the layer of material and the nanotubes are made of the same material.

12. The device of claim 10 , wherein the fluidic passage comprises a microfluidic device.

13. The device of claim 10 , further comprising a holding structure, wherein the porous structure is a bottom surface of the holding structure, wherein walls form side boundaries of the holding structure, and wherein the nanotubes extending above the porous structure surface extend up into an area bounded by the walls and the porous structure.

14. The device of claim 10 , wherein the porous structure is made of a material that is different than the nanotubes.

15. The device of claim 10 , wherein the distance is about 100 nm to 10 μm.

16. The device of claim 10 , wherein the porous structure is made of a material selected from the group consisting of: polycarbonate, polyester, a polymer, an etchable material that can be processed with pores, silicon, and a combination thereof.

17. The device of claim 10 , wherein the nanotubes are made of a material selected from the group consisting of: alumina, TiO 2 , SnO 2 , ZrO 2 , ZnO 2 , carbon, a nitride, platinum, gold, silver, indium tin oxide (ITO), SiO 2 , Ni, NiO, and a combination thereof.

18. The device of claim 10 , wherein the porous structure has a thickness of about 100 nm to 50 μm.

Assignees

• Vandersarl Jules J
• Xu Alexander M
• Melosh Nicholas A
• Tayebi Noureddine
• Univ Leland Stanford Junior

Inventors

• Vandersarl Jules J
• Xu Alexander M
• Melosh Nicholas A
• Tayebi Noureddine

Classifications

• C12N2533/30

  Synthetic polymers · CPC title

• C12N2521/00

  Culture process characterised by the use of hydrostatic pressure, flow or shear forces · CPC title

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title

• C12N2535/00

  Supports or coatings for cell culture characterised by topography · CPC title

• B82Y5/00 Primary

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title