Pertussis antibodies and uses thereof

Patent metadata

Field	Value
Publication number	US-9260510-B2
Application number	US-201314135353-A
Country	US
Kind code	B2
Filing date	Dec 19, 2013
Priority date	Sep 17, 2010
Publication date	Feb 16, 2016
Grant date	Feb 16, 2016

Abstract

Compositions and methods are provided that are useful to treat respiratory diseases such as whooping cough. Further, compositions and methods of immunizing are provided.

First claim

The invention claimed is:

1. A humanized mouse 1B7 antibody capable of binding a pertussis toxin protein, the humanized antibody comprising a humanized heavy chain and a humanized light chain, wherein said humanized light chain comprises a phenylalanine at a position corresponding to Kabat position 65, a mouse CDR L1 as set forth in SEQ ID NO:3, a mouse CDR L2 as set forth in SEQ ID NO:4, and a mouse CDR L3 as set forth in SEQ ID NO:5; and
wherein said humanized heavy chain comprises a mouse CDR H1 as set forth in SEQ ID NO:6, a mouse CDR H2 as set forth in SEQ ID NO:7 and a mouse CDR H3 as set forth in SEQ ID NO:8.

2. The humanized mouse 1B7 antibody of claim 1 , wherein the antibody is a Fab′ fragment.

3. The humanized mouse 1B7 antibody of claim 1 , wherein the antibody comprises a human constant region.

4. The humanized mouse 1B7 antibody of claim 1 , wherein the antibody is an IgG.

5. The humanized mouse 1B7 antibody of claim 1 , wherein the antibody is a single chain antibody (scFv).

6. The humanized mouse 1B7 antibody of claim 1 , wherein the antibody is capable of binding said pertussis toxin protein with an equilibrium dissociation constant (K D ) less than 10 nM.

7. The humanized mouse 1B7 antibody of claim 6 , wherein said pertussis toxin protein is a truncated pertussis toxin protein.

8. The humanized mouse 1B7 antibody of claim 7 , wherein said truncated pertussis toxin protein comprises the amino acid sequence corresponding to amino acid residues 1 to 220 of SEQ ID NO:9.

9. The humanized mouse 1B7 antibody of claim 6 , wherein the humanized antibody is an antibody which competes with an antibody that is capable of binding a pertussis toxin protein comprising the amino acid sequence corresponding to amino acid residues 1 to 220 of SEQ ID NO:9.

10. A pharmaceutical composition comprising a therapeutically effective amount of a humanized mouse 1B7 antibody as set forth in claim 1 .

11. A method of treating whooping cough in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a humanized mouse 1B7 antibody as set forth in claim 1 .

Assignees

• Univ Texas

Inventors

• Maynard Jennifer A
• Sutherland Jamie

Classifications

• C07K2317/92

  Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value · CPC title

• C07K2317/76

  Antagonist effect on antigen, e.g. neutralization or inhibition of binding · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• A61P37/04

  Immunostimulants · CPC title

• C07K16/1225 Primary

  from Bordetella (G) · CPC title