Protein having immunogenicity of cedar pollen, polynucleotide encoding the protein, and use thereof

Patent metadata

Field	Value
Publication number	US-9260491-B2
Application number	US-201013504636-A
Country	US
Kind code	B2
Filing date	Oct 28, 2010
Priority date	Oct 30, 2009
Publication date	Feb 16, 2016
Grant date	Feb 16, 2016

Abstract

It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.

First claim

The invention claimed is:

1. A protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence encoded by a polynucleotide comprising the nucleotide sequence represented by SEQ ID NO: 5; and
(B) a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence encoded by a polynucleotide comprising the nucleotide sequence represented by SEQ ID NO: 5, and having immunogenicity to cedar pollen.

2. A polynucleotide selected from the group consisting of:
(a) a polynucleotide comprising the nucleotide sequence represented by SEQ ID NO: 5; and
(b) a polynucleotide encoding a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence encoded by a polynucleotide comprising the nucleotide sequence represented by SEQ ID NO: 5, and having the immunogenicity to the cedar pollen.

3. A vector, comprising the polynucleotide according to claim 2 .

4. A transformant in which the vector according to claim 3 has been introduced, wherein said transformant is a microorganism, animal cell, plant, or plant cell.

5. The transformant according to claim 4 , wherein said transformant is a plant.

6. The transformant according to claim 4 , wherein said transformant is a gramineous plant.

7. A method for producing a protein, the method comprising expressing the polynucleotide of the transformant according to claim 4 and collecting the protein encoded by said expressed polynucleotide.

8. A method for imparting an immunogenicity of a cedar pollen to a plant, the method comprising introducing the polynucleotide according to claim 2 into the plant.

9. A method for producing a plant body having an immunogenicity of a cedar pollen, the method comprising:
introducing a vector comprising the polynucleotide according to claim 2 into a plant organ, a plant tissue, or a plant cell to obtain a transformant; and
growing the plant body from said transformant.

10. A therapeutic agent or a prophylactic agent for a cedar pollinosis, comprising the protein according to claim 1 as an active ingredient.

11. A method for treating or preventing a cedar pollinosis, the method comprising administering the protein according to claim 1 to a human in need thereof.

12. A therapeutic agent or a prophylactic agent for a cedar pollinosis, comprising the transformant according to claim 5 as an active ingredient.

13. A method for treating or preventing a cedar pollinosis, the method comprising administering the transformant according to claim 5 to a human in need thereof.

Assignees

• Takaiwa Fumio
• Takagi Hidenori
• Wakasa Yuhya
• Fujii Yuji
• Shinya Tomotaka
• Kasahara Saori
• Jujo Paper Co Ltd
• Nat Inst Of Agrobio Sciences

Inventors

• Takaiwa Fumio
• Takagi Hidenori
• Wakasa Yuhya
• Fujii Yuji
• Shinya Tomotaka
• Kasahara Saori

Classifications

• C07K14/415 Primary

  from plants · CPC title

• A61K39/36

  from pollen · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title