Method for separating cell from biological tissue
US-2015368615-A1 · Dec 24, 2015 · US
Process for making a milk-based protein hydrolysate
US9259023B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9259023-B2 |
| Application number | US-75220710-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 1, 2010 |
| Priority date | Apr 2, 2009 |
| Publication date | Feb 16, 2016 |
| Grant date | Feb 16, 2016 |
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- Title
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Abstract
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The present invention relates to an enzymatic process for making a milk-based protein hydrolysate and use of such hydrolysate, e.g., in an infant formula composition.
First claim
Opening claim text (preview).
The invention claimed is: 1. A process for the preparation of a milk-based protein hydrolysate comprising treatment of a solution of a milk-based proteinaceous material with a) a trypsin-like endopeptidase produced from a microorganism and comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO: 2, wherein the trypsin-like endopeptidase is from a strain of Fusarium , and b) at least one other endopeptidase produced from a microorganism and comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO: 8, wherein the at least one other endopeptidase is from a strain of Nocardiopsis. 2. The process of claim 1 , wherein the ratio of trypsin-like endopeptidase to milk-based protein is 0.01-10% weight/weight. 3. The process of claim 1 , wherein the ratio of the at least one other endopeptidase to milk-based protein is 0.001-1% weight/weight. 4. The process of claim 1 , comprising adding the at least one other endopeptidase at a concentration which is between 2% and 50% of the concentration of trypsin-like endopeptidase based on the weight of the endopeptidases. 5. The process of claim 1 , comprising conducting hydrolysis at a temperature of about 40° C. to 60° C., during 1 to 6 hours, at pH values within the range 6.5 to 8.5. 6. The process of claim 1 , further comprising adding the milk-based protein hydrolysate obtained into an infant formula composition. 7. The process of claim 1 , wherein the trypsin-like endopeptidase is a polypeptide comprising an amino acid sequence having at least 98% identity to the mature polypeptide of SEQ ID NO: 2. 8. The process of claim 1 , wherein the trypsin-like endopeptidase is a polypeptide comprising an amino acid sequence having at least 98% identity to the mature polypeptide of SEQ ID NO: 8.
Assignees
Inventors
Classifications
derived from fungi · CPC title
Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula · CPC title
Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby (A23C9/18 takes precedence) · CPC title
Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4) · CPC title
Dairy proteins · CPC title
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Frequently asked questions
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- What does patent US9259023B2 cover?
- The present invention relates to an enzymatic process for making a milk-based protein hydrolysate and use of such hydrolysate, e.g., in an infant formula composition.
- Who is the assignee on this patent?
- Oestergaard Peter Rahbek, Ernst Steffen, Lynglev Gitte B, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/52. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 16 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).