Mixing of fluids in fluidic systems

Patent metadata

Field	Value
Publication number	US-9255866-B2
Application number	US-201414446828-A
Country	US
Kind code	B2
Filing date	Jul 30, 2014
Priority date	Mar 13, 2013
Publication date	Feb 9, 2016
Grant date	Feb 9, 2016

Abstract

Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.

First claim

What is claimed is:

1. A method, comprising:
flowing in a channel a series of fluid plugs comprising a first fluid plug comprising a first fluid, a second fluid plug comprising a second fluid, and a third fluid plug comprising a third fluid, wherein the first fluid plug has a first volume,
wherein the second fluid plug is positioned between the first and third fluid plugs, and
wherein the second fluid is immiscible with each of the first and third fluids,
wherein prior to the flowing step, the channel comprises a reagent that is dry, the method comprising:
contacting the first and/or third fluid with the reagent;
while the first fluid plug is flowing, depositing at least a portion of the first fluid on a wall of the channel for combining with the third fluid plug;
reducing the first volume of the first fluid plug by at least 50%;
combining at least a portion of the first fluid into the third fluid plug so as to mix at least portions of the first and third fluids; and
after the combining step, flowing in the channel the first fluid plug, the second fluid plug, and the third fluid plug which comprises the third fluid and at least a portion of the first fluid.

2. A method as in claim 1 , comprising contacting the reagent with the first and/or third fluid, and performing a chemical and/or biological reaction and/or a binding event between the reagent and an at least one component of the first and/or third fluid.

3. A method as in claim 1 , comprising reducing the first volume of the first fluid plug by at least 75%.

4. A method as in claim 1 , wherein after the step of flowing in the channel the first fluid plug, the second fluid plug, and the third fluid plug which comprises the third fluid and at least a portion of the first fluid, the method comprises reducing the first volume of the first fluid plug by 100%.

5. A method as in claim 1 , wherein the first fluid and the third fluid are miscible with one another.

6. A method as in claim 1 , wherein the first fluid plug comprises a first component and the third fluid plug comprises a second component, the method comprising performing one or more chemical and/or biological reactions and/or binding events involving each of the first and second components.

7. A method as in claim 6 , comprising performing a chemical and/or biological reaction and/or a binding event between the first and second components.

8. A method as in claim 1 , flowing in series a fourth fluid plug comprising a fourth fluid and a fifth fluid plug comprising a fifth fluid along with the first, second and third fluid plugs, wherein the fourth fluid is immiscible with the first, third and fifth fluids.

9. A method as in claim 1 , comprising reducing the volume of the third fluid plug.

10. A method as in claim 1 , comprising reducing the volume of the third fluid plug by at least 50%.

11. A method as in claim 8 , comprising combining at least a portion of the third fluid into the fifth fluid plug so as to mix at least portions of the third and fifth fluids.

12. A method as in claim 1 , comprising depositing at least a portion of the first fluid on a wall of the channel during the step of flowing the first, second and third fluid plugs in series.

13. A method as in claim 12 , wherein the first fluid is deposited as a film on the wall of the channel.

14. A method as in claim 12 , wherein the first fluid is deposited as fluid droplets on the wall of the channel.

15. A method as in claim 1 , wherein the first and/or third fluid comprises a metal salt.

16. A method as in claim 1 , wherein the second fluid comprises a gas.

17. A method as in claim 1 , wherein the second fluid is hydrophobic.

18. A method as in claim 1 , wherein the second fluid is directly adjacent the first and third fluids.

19. A method as in claim 1 , wherein the channel has a cross-section comprising a radius of curvature smaller than the half-width of the channel.

20. A method as in claim 1 , wherein the channel has a cross-section comprising a radius of curvature smaller than the half-height of the channel.

21. A method as in claim 1 , wherein the channel has an average diameter of less than or equal to 1 mm.

22. A method as in claim 1 , wherein the combining step is performed while the first, second, and third fluid plugs are flowing in series from an upstream portion to a downstream portion of the channel.

23. A method as in claim 1 , wherein after the combining step, the first, second, and third fluid plugs are flowed in series from an upstream portion to a downstream portion of the channel.

24. A method as in claim 1 , wherein the first and/or third fluid comprises a wetting agent.

25. A method as in claim 1 , wherein the combining step occurs while the first fluid plug, the second fluid plug, and the third fluid plug are flowing in series.

26. A method as in claim 1 , wherein the channel is configured to remove at least a portion of the first fluid from the first fluid plug and deposit at least a portion of the first fluid on a wall of the channel during the step of flowing.

Assignees

• Opko Diagnostics Llc

Inventors

• Dirckx Matthew
• Linder Vincent
• Taylor Jason

Classifications

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• B01L2400/0694

  vents used to stop and induce flow, backpressure valves · CPC title

• G01N35/08

  using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L3/502746

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title