Assay and other reactions involving droplets
US-2015353999-A1 · Dec 10, 2015 · US
Integrated capture and amplification of target nucleic acid for sequencing
US9255293B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9255293-B2 |
| Application number | US-201113879572-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 1, 2011 |
| Priority date | Nov 1, 2010 |
| Publication date | Feb 9, 2016 |
| Grant date | Feb 9, 2016 |
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- Title
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Abstract
Official abstract text for this publication.
The invention provides efficient methods of preparing a target nucleic acid in a form suitable for sequencing. The methods are particularly amenable for preparing high quality nucleic acids for massively parallel sequencing. The methods involve capturing a target nucleic acid from a sample and PCR amplification of the target nucleic acid. The target nucleic acid is captured by binding to a capture probe, which in turn binds to an immobilized probe. The immobilized probe is typically immobilized via a magnetic bead. The captured target nucleic acid is PCR amplified by thermocycling without prior dissociation of the target nucleic acid from the beads. The efficiency of the method lies in part in that both the capture and amplification steps are performed in a single vessel. The amplified nucleic acid can then be sequenced.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of preparing a target nucleic acid, comprising contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that hybridizes to the target nucleic acid and a second segment that hybridizes to the immobilized probe, wherein the target nucleic acid hybridizes to the first segment of the capture probe, and the second segment of the capture probe hybridizes to the immobilized probe, thereby…
Assignees
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Classifications
- C12Q1/6834Primary
Chemistry & Metallurgy · mapped topic
- C12Q1/6874Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US9255293B2 cover?
- The invention provides efficient methods of preparing a target nucleic acid in a form suitable for sequencing. The methods are particularly amenable for preparing high quality nucleic acids for massively parallel sequencing. The methods involve capturing a target nucleic acid from a sample and PCR amplification of the target nucleic acid. The target nucleic acid is captured by binding to a capt…
- Who is the assignee on this patent?
- Pollner Reinhold, Lee Shyun-Shyun, Gen Probe Inc
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6834. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 09 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).