Polynucleotide encoding PPAT derived from jatropha and use thereof

Patent metadata

Field	Value
Publication number	US-9255278-B2
Application number	US-201113643396-A
Country	US
Kind code	B2
Filing date	Oct 25, 2011
Priority date	Dec 8, 2010
Publication date	Feb 9, 2016
Grant date	Feb 9, 2016

Abstract

A PPAT polypeptide of SEQ ID NO: 1 derived from Jatropha , a PPAT polynucleotide of SEQ ID NO: 2 and so on were found. By transforming Jatropha with these PPAT polynucleotides, it is possible to overexpress the PPAT polypeptide in comparison with a wild type, and biosynthesis of coenzyme A is promoted by these polypeptides, the metabolic function and viability of the transformed Jatropha are enhanced, and for example, stress resistance can be significantly improved.

First claim

The invention claimed is:

1. A vector comprising:
(a) a cDNA comprising the polynucleotide represented by SEQ ID NO: 2 or
(b) a polynucleotide encoding a phosphopantetheine adenylyltransferase (PPAT) polypeptide having a homology of 95% or higher with the amino acid sequence of SEQ ID NO: 1, wherein said polynucleotide is operably linked to a heterologous regulatory element.

2. A cDNA comprising the polynucleotide represented by SEQ ID NO: 2.

3. An isolated PPAT polypeptide other than the polypeptide of SEQ ID NO: 1, wherein said polypeptide has an amino acid sequence having a homology of 95% or higher with the amino acid sequence of SEQ ID NO: 1, and wherein the polypeptide catalyzes an exchange reaction between 4′-phosphopantetheine and dephospho-coenzyme A.

4. A polynucleotide encoding the polypeptide according to claim 3 .

5. A The vector according to claim 1 , wherein said vector is a Jatropha plant transformation vector.

6. A transformant containing the vector according to claim 5 .

7. A transgenic Jatropha plant transformed with the vector according to claim 5 , wherein said transgenic plant has increased stress tolerance when compared to a corresponding control Jatropha plant.

8. A transgenic seed harvested from the stress resistant transgenic Jatropha plant according to claim 7 , wherein the seed comprises the vector.

9. A method of producing a Jatropha oil, comprising: squeezing the seed according to claim 8 to obtain a material oil, and purifying the material oil.

10. A method of producing a stress resistant Jatropha plant, comprising:
producing an isolated polynucleotide selected from the following polynucleotides:
(a) a cDNA comprising the polynucleotide represented by SEQ ID NO: 2; or
(b) a polynucleotide encoding a PPAT polypeptide having a homology of 95% or higher with the amino acid sequence of SEQ ID NO: 1;

producing a Jatropha plant transformation vector comprising said isolated polynucleotide; and
transforming a Jatropha plant with said vector.

Assignees

• Fukui Kiichi
• Cartagena Joyce
• Wada Naoki
• Kohinata Tsutomu
• Yushio Yasuhisa
• Ikeguchi Naoki
• Tabata Satoshi
• Sumitomo Electric Industries
• Univ Osaka

Inventors

• Fukui Kiichi
• Cartagena Joyce
• Wada Naoki
• Kohinata Tsutomu
• Yushio Yasuhisa
• Ikeguchi Naoki
• Tabata Satoshi

Classifications

• C12N9/1241

  Nucleotidyltransferases (2.7.7) · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12Y207/07003

  Pantetheine-phosphate adenylyltransferase (2.7.7.3) · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title