Polynucleotides encoding Toll-Like Receptor 3 antagonists

Patent metadata

Field	Value
Publication number	US-9255153-B2
Application number	US-201313777513-A
Country	US
Kind code	B2
Filing date	Feb 26, 2013
Priority date	Oct 31, 2008
Publication date	Feb 9, 2016
Grant date	Feb 9, 2016

Abstract

Toll Like Receptor 3 (TLR3) antibody antagonists, polynucleotides encoding TLR3 antibody antagonists or fragments thereof, and methods of making and using the foregoing are disclosed.

First claim

The invention claimed is:

1. An isolated polynucleotide encoding an antibody heavy chain variable region (VH) comprising the heavy chain complemetarity determining regions (HCDR) 1, 2 and 3 (HCDR1, HCDR2 and HCDR3) amino acid sequences as shown in SEQ ID NOs: 82, 196 and 84, wherein the HCDR2 of SEQ ID NO: 196 is further defined as shown in Formula (V):

(V)

Xaa 24 -I-D-P-S-D-S-Y-T-N-Y-Xaa 25 -P-S-F-Q-G,

wherein
Xaa 24 may be Phe or Arg; and
Xaa 25 may be Ala or Ser.

2. The isolated polynucleotide of claim 1 , wherein the HCDR2 comprises the amino acid sequence as shown in SEQ ID NOs: 83, 86, 112, 114, 117 or 118.

3. The isolated polynucleotide of claim 1 , wherein the VH comprises the amino acid sequence as shown in SEQ ID NOs: 38, 40, 42, 124-129 or 216.

4. An isolated polynucleotide encoding an antibody light chain variable region (VL) comprising the light chain complementarity determining regions (LCDR) 1, 2 and 3 (LCDR1, LCDR2 and LCDR3) amino acid sequences as shown in SEQ ID NOs: 79, 80 and 195, wherein the LCDR3 of SEQ ID NO: 193 is further defined as shown in Formula (VI):

(VI)

Q-Q-Xaa 18 -Xaa 19 -Xaa 20 -Xaa 21 -Xaa 22 -Xaa 23 -T,

wherein
Xaa 18 may be Tyr, Gly or Ala;
Xaa 19 may be Gly, Glu or Asn;
Xaa 20 may be Ser or Thr;
Xaa 21 may be Val, Ile or Leu;
Xaa 22 may be Ser or Leu; and
Xaa 23 may be Ile, Ser, Pro or Tyr.

5. The isolated polynucleotide of claim 4 , wherein the LCDR3 comprises the amino acid sequence as shown in SEQ ID NOs: 81, 85, 87 or 113.

6. The isolated polynucleotide of claim 5 , wherein the VL comprises the amino acid sequence as shown in SEQ ID NOs: 37, 39, 41, 122 or 123.

7. A vector comprising the isolated polynucleotide as in any one of claims 1 - 6 .

8. An isolated host cell comprising the vector of claim 7 .

9. A method of making an antibody that binds toll-like receptor 3 (TLR3) comprising culturing the host cell of claim 8 and recovering the antibody produced by the host cell.

Assignees

• Cunningham Mark
• Feng Yiqing
• Heeringa Katharine
• Luo Jinquan
• Rauchenberger Robert
• Rutz Mark
• San Mateo Lani
• Sarisky Robert
• Sweet Raymond
• Teng Fang
• Teplyakov Alexey
• Wu Sheng-Jiun
• Janssen Biotech Inc

Inventors

• Cunningham Mark
• Feng Yiqing
• Heeringa Katharine
• Luo Jinquan
• Rauchenberger Robert
• Rutz Mark
• San Mateo Lani
• Sarisky Robert
• Sweet Raymond
• Teng Fang
• Teplyakov Alexey
• Wu Sheng-Jiun

Classifications

• A61P37/00

  Drugs for immunological or allergic disorders · CPC title

• A61P7/00

  Drugs for disorders of the blood or the extracellular fluid · CPC title

• A61P37/06

  Immunosuppressants, e.g. drugs for graft rejection · CPC title

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P37/02

  Immunomodulators · CPC title