Alteration of cell membrane for new functions using IL-2 and streptavidin

Patent metadata

Field	Value
Publication number	US-9255133-B2
Application number	US-201414280940-A
Country	US
Kind code	B2
Filing date	May 19, 2014
Priority date	Jun 30, 2000
Publication date	Feb 9, 2016
Grant date	Feb 9, 2016

Abstract

Methods and compositions are provided for the persistent modification of cell membranes with exogenous proteins so as to alter the function of the cell to achieve effects similar to those of gene therapy, without the introduction of exogenous DNA. DNA sequences, the proteins and polypeptides embodying these sequences are disclosed for modulating the immune system. The modulations include down-regulation, up-regulation and apoptosis.

First claim

What is claimed:

1. A chimeric protein comprising IL-2 (Interleukin-2), wherein the chimeric protein is encoded by a nucleic acid comprising SEQ ID NO:4.

2. A cell having a cell surface attached to which is a chimeric protein according to claim 1 .

3. A vaccine comprising a tumor cell having a cell surface attached to which is a chimeric protein according to claim 1 .

4. A method of attaching IL-2 (Interleukin-2) to a cell surface, comprising contacting a biotinylated cell with a chimeric protein according to claim 1 .

5. The method of claim 4 , wherein the method is effected by administering the chimeric protein to a subject containing the biotinylated cell.

6. The method of claim 4 , further comprising the step of biotinylating a cell ex vivo to form the biotinylated cell.

7. The method of claim 4 , wherein the cell is selected from the group consisting of splenocytes, tumor cells, bone marrow cells, endothelial cells, islet cells and T cells.

8. A method of modulating the immune system of a subject in need thereof, comprising administering to the subject a chimeric protein according to claim 1 .

9. The method of claim 8 , wherein the subject is suffering from cancer or infectious disease.

10. An isolated nucleic acid having a nucleotide sequence comprising SEQ ID NO:4.

Assignees

• Univ Louisville Res Found

Inventors

• Shirwan Haval

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• C07K14/46

  from vertebrates · CPC title

• A61K38/2013

  IL-2 · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• Y10S530/816

  Attached to the carrier via a bridging agent · CPC title