Near-infrared fluorescent dyes with large stokes shifts

Patent metadata

Field	Value
Publication number	US-9250246-B2
Application number	US-201214129223-A
Country	US
Kind code	B2
Filing date	Jun 29, 2012
Priority date	Jun 29, 2011
Publication date	Feb 2, 2016
Grant date	Feb 2, 2016

Abstract

Embodiments of near-infrared (NIR) dyes are disclosed, along with methods and kits for detecting analytes with the NIR dyes. The NIR dyes have a structure according to the general structure

At least one of R 1 /R 2 , R 2 /R 3 , R 3 /R 4 , R 5 /R 6 , R 6 /R 7 , and/or R 7 /R 8 together forms a substituted or unsubstituted cycloalkyl or aryl.

First claim

We claim:

1. A compound having a chemical structure selected from

2. A method for selectively detecting an analyte in a biological fluid, comprising:
combining a compound according to claim 1 with a biological fluid to form a solution;
exposing the solution to a light source; and
detecting the analyte by detecting fluorescence from the compound.

3. The method of claim 2 where detecting fluorescence from the compound comprises detecting fluorescence at a wavelength corresponding to an emission spectrum maximum of the compound, the method further comprising quantitating the analyte by measuring an amount of fluorescence from the compound at a wavelength corresponding to an emission spectrum maximum of the compound.

4. The method of claim 2 where the biological fluid comprises blood or urine.

5. A kit for detecting an analyte, comprising at least one compound according to claim 1 , wherein the compound when combined with a sample comprising the analyte will undergo a change in its absorbance spectrum and/or emission spectrum compared to the compound in a sample that does not comprise the analyte.

6. The kit of claim 5 , further comprising at least one buffer solution in which the compound when combined with a sample comprising the analyte will undergo a change in its absorbance spectrum and/or emission spectrum compared to the compound combined with the buffer solution and a sample that does not comprise the analyte.

Assignees

• Strongin Robert Michael
• Sibrian-Vazquez Martha
• Escobedo-Cordova Jorge Omar
• Lowry Mark Allen
• Univ Portland State

Inventors

• Strongin Robert Michael
• Sibrian-Vazquez Martha
• Escobedo-Cordova Jorge Omar
• Lowry Mark Allen

Classifications

• C09B11/12

  without any OH group bound to an aryl nucleus · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N33/49

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• C07D311/82 Primary

  Xanthenes · CPC title

• C07D493/10

  Spiro-condensed systems · CPC title