Raman spectrum measuring method for drug inspection

Patent metadata

Field	Value
Publication number	US-9250180-B2
Application number	US-201414495233-A
Country	US
Kind code	B2
Filing date	Sep 24, 2014
Priority date	Sep 25, 2013
Publication date	Feb 2, 2016
Grant date	Feb 2, 2016

Abstract

A raman spectrum measuring method for drug inspection is provided, comprising: measuring raman spectrum of a sample to be inspected to acquire an original raman spectrum curve of the sample; determining whether the original raman spectrum curve has a characterizing portion, and if not, measuring a mixture of the sample and an enhancing agent to acquire an enhanced raman spectrum curve of the sample; and if the original raman spectrum curve of the sample to be inspected has a characterizing portion, comparing the original raman spectrum curve of the sample with data in an original raman spectrum database of a drug to determine whether the sample contains the drug, otherwise, comparing the enhanced raman spectrum curve of the sample with data in an enhanced raman spectrum database of the drug to determine whether the sample to be inspected contains the drug.

First claim

What the claims is:

1. A raman spectrum measuring method for drug inspection, comprising the steps of:
(a) measuring raman spectrum of a sample to be inspected to acquire an original raman spectrum curve of the sample to be inspected;
(b) determining whether the original raman spectrum curve of the sample to be inspected has a characterizing portion or not, and if not, measuring a mixture of the sample to be inspected and an enhancing agent to acquire an enhanced raman spectrum curve of the sample to be inspected; and
(c) if the original raman spectrum curve of the sample to be inspected has a characterizing portion, comparing the original raman spectrum curve of the sample to be inspected with data in an original raman spectrum database of a drug to determine whether the sample to be inspected contains the drug, otherwise, if the original raman spectrum curve of the sample to be inspected does not have the characterizing portion, comparing the enhanced raman spectrum curve of the sample to be inspected with data in an enhanced raman spectrum database of a drug to determine whether the sample to be inspected contains the drug.

2. The raman spectrum measuring method for drug inspection of claim 1 , further comprising before step (a), the steps:
(o1) measuring raman spectrum of a drug sample to acquire an original raman spectrum curve of the drug sample;
(o2) determining whether the original raman spectrum curve of the drug sample has the characterizing portion or not, and if the original raman spectrum curve of the drug sample has the characterizing portion, establishing an original raman spectrum database of the drug based on the original raman spectrum curve of the drug sample, otherwise, if the original raman spectrum curve of the drug sample does not have the characterizing portion, measuring a mixture of the drug sample and the enhancing agent to acquire an enhanced raman spectrum curve of the drug sample and establishing the enhanced raman spectrum database of the drug based on the enhanced raman spectrum curve of the drug sample.

3. The raman spectrum measuring method for drug inspection of claim 1 , wherein, in step (c), the step of comparing the original raman spectrum curve of the sample to be inspected with data in the original raman spectrum database of the drug is performed by calculating a similarity between the original raman spectrum curve of the sample to be inspected and the original raman spectrum curve of the drug sample, and the step of comparing the enhanced raman spectrum curve of the sample to be inspected with data in the enhanced raman spectrum database of the drug is performed by calculating a similarity between the enhanced raman spectrum curve of the sample to be inspected and the enhanced raman spectrum curve of the drug sample, and if the similarity between the original raman spectrum curve of the sample to be inspected and the original raman spectrum curve of the drug sample is greater than a first threshold or the similarity between the enhanced raman spectrum curve of the sample to be inspected and the enhanced raman spectrum curve of the drug sample is greater than a second threshold, it is determined that the sample to be inspected contains the drug.

4. The raman spectrum measuring method for drug inspection of claim 3 , wherein a characterizing portion of the raman spectrum curves is one or more characterizing peak(s) and the similarity is calculated in weight on basis of peak position(s), peak width(s) and/or peak height(s) of the characterizing peak(s).

5. The raman spectrum measuring method for drug inspection of claim 1 , wherein the mixture of the sample to be inspected and the enhancing agent is formed by directly mixing the sample to be inspected with the enhancing agent or mixing the enhancing agent with an aqueous or organic solution of the sample to be inspected, and wherein the mixture of the drug sample and the enhancing agent is formed by directly mixing the drug sample with the enhancing agent or mixing the enhancing agent with an aqueous or organic solution of the drug sample.

6. The raman spectrum measuring method for drug inspection of claim 1 , wherein the enhancing agent contains any one of metal nanoparticles, metal nanolines, metal nanoclusters, carbon nanotubes or carbon nanoparticles having a size in a range of 1-1000 nm or any combination thereof.

7. The raman spectrum measuring method for drug inspection of claim 1 , wherein the enhancing agent comprises metal nanomaterials.

8. The raman spectrum measuring method for drug inspection of claim 7 , wherein the enhancing agent further comprises chloride ions, bromine ions, sodium ions, potassium ions and/or sulfate radical ions.

9. The raman spectrum measuring method for drug inspection of claim 6 , wherein the metal comprises any one of gold, silver, copper, magnesium, aluminium, iron, cobalt, nickel, palladium or platinum, or any combination thereof.

10. The raman spectrum measuring method for drug inspection of claim 7 , wherein the metal comprises any one of gold, silver, copper, magnesium, aluminium, iron, cobalt, nickel, palladium or platinum, or any combination thereof.

11. The raman spectrum measuring method for drug inspection of claim 8 , wherein the metal comprises any one of gold, silver, copper, magnesium, aluminium, iron, cobalt, nickel, palladium or platinum, or any combination thereof.

Assignees

• Nuctech Co Ltd
• Univ Tsinghua

Inventors

• Chen Zhiqiang
• Zhang Li
• Zhang Jianhong
• Wang Hongqiu
• Zhao Ziran
• Yi Yumin
• Gu Jianping
• Huang Qingping

Classifications

• G01N21/658

  enhancement Raman, e.g. surface plasmons · CPC title

• G01N21/65

  Raman scattering · CPC title

• G01J3/44

  Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} · CPC title

• G01N33/15 Primary

  Medicinal preparations {; Physical properties thereof, e.g. dissolubility} · CPC title

• G01N21/47 Primary

  Scattering, i.e. diffuse reflection (G01N21/25, G01N21/41 take precedence {G01N21/55 takes precedence}) · CPC title