Microorganism having high squalene-producing ability, and method for producing squalene by means of same

Patent metadata

Field	Value
Publication number	US-9249429-B2
Application number	US-201113992638-A
Country	US
Kind code	B2
Filing date	Dec 9, 2011
Priority date	Dec 9, 2010
Publication date	Feb 2, 2016
Grant date	Feb 2, 2016

Abstract

Provided is a strain belonging to the genus Aurantiochytrium , the strain being characterized by there being at least 10 mass % of squalene in all the lipids produced by the strain, and by forming colonies exhibiting an orange-to-red color.

First claim

What is claimed is:

1. A method for producing squalene that employs a strain of Aurantiochytrium tsukuba-3 (deposit number: FERM BP-11442), wherein said strain produces at least 10 mass % squalene among the total lipids, and forming colonies that exhibit an orange-to-red color, said method comprising the steps of:
culturing cells of said strain at pH of 4.5-9.0 and 10° C. to 30° C. for 3 to 7 days;
harvesting squalene-containing lipids from said cultured cells; and then
extracting squalene from said squalene-containing lipids.

2. The method of claim 1 , wherein said culturing step is conducted in artificial sea water.

Assignees

• Watanabe Makoto
• Kaya Kunimitsu
• Shiho Makato
• Nakazawa Atsushi
• Inoue Isao
• Honda Daisuke
• Univ Tsukuba

Inventors

• Watanabe Makoto
• Kaya Kunimitsu
• Shiho Makato
• Nakazawa Atsushi
• Inoue Isao
• Honda Daisuke

Classifications

• C12P5/026 Primary

  Unsaturated compounds, i.e. alkenes, alkynes or allenes · CPC title

• C12R1/89

  Chemistry & Metallurgy · mapped topic

• C12R2001/89

  Algae {; Processes using algae} · CPC title

• C12N1/125

  Unicellular algae isolates · CPC title